Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein

dc.contributor.authorPereira, Catia Mendes [UNIFESP]
dc.contributor.authorYamauchi, Lucy M.
dc.contributor.authorLevin, Mariano J.
dc.contributor.authorSilveira, José Franco da
dc.contributor.authorCastilho, Beatriz A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionIngebi
dc.date.accessioned2016-01-24T12:30:37Z
dc.date.available2016-01-24T12:30:37Z
dc.date.issued1998-07-01
dc.description.abstractThe JL8 protein antigen from Trypanosoma cii , a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the LamB protein of Escherichia coli as a carl icr of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the LamB protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. the fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationIngebi, RA-1428 Buenos Aires, DF, Argentina
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent125-131
dc.identifierhttp://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.x
dc.identifier.citationFems Microbiology Letters. Amsterdam: Elsevier B.V., v. 164, n. 1, p. 125-131, 1998.
dc.identifier.doi10.1111/j.1574-6968.1998.tb13077.x
dc.identifier.issn0378-1097
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25920
dc.identifier.wosWOS:000074571400019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFems Microbiology Letters
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectimmunodominant repetitive antigenen
dc.subjectB cell epitope mappingen
dc.subjectEscherichia coli LamB proteinen
dc.subjectChagas diseaseen
dc.titleMapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB proteinen
dc.typeArtigo
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