Effects of A1 receptor agonist/antagonist on spontaneous seizures in pilocarpine-induced epileptic rats

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dc.citation.volume61
dc.contributor.authorAmorim, Beatriz Oliveira
dc.contributor.authorHamani, Clement
dc.contributor.authorFerreira, Elenn
dc.contributor.authorMiranda, Maisa Ferreira
dc.contributor.authorFernandes, Maria Jose S.
dc.contributor.authorRodrigues, Antonio M.
dc.contributor.authorde Almeida, Antonio-Carlos G.
dc.contributor.authorCovolan, Luciene
dc.coverageSan Diego
dc.date.accessioned2020-08-14T13:43:58Z
dc.date.available2020-08-14T13:43:58Z
dc.date.issued2016
dc.description.abstractAdenosine is an endogenous anticonvulsant that activates pre- and postsynaptic adenosine A(1) receptors. A(1) receptor agonists increase the latency for the development of seizures and status epilepticus following pilocarpine administration. Although hippocampal adenosine is increased in the chronic phase of the pilocarpine model, it is not known whether the modulation of A(1) receptors may influence the frequency of spontaneous recurrent seizures (SRS). Here, we tested the hypothesis that the A(1) receptor agonist RPia ([R]-N-phenylisopropyladenosine) and the A(1) antagonist DPCPX (8-Cyclopentyl-1,3-dipropylxanthine) administered to chronic pilocarpine epileptic rats would respectively decrease and increase the frequency of SRS and hippocampal excitability. Four months after Pilo-induced SE, chronic epileptic rats were video-monitored for the recording of SRS before (basal) and after a 2-week treatment with RPia (25 mu g/kg) or DPCPX (50 mu g/kg). Following sacrifice, brain slices were studied with electrophysiology. We found that rats given RPia had a 93% nonsignificant reduction in the frequency of seizures compared with their own pretreatment baseline. In contrast, the administration of DPCPX resulted in an 87% significant increase in seizure rate. Nontreated epileptic rats had a similar frequency of seizures along the study. Corroborating our behavioral data, in vitro recordings showed that slices from animals previously given DPCPX had a shorter latency to develop epileptiform activity, longer and higher DC shifts, and higher spike amplitude compared with slices from nontreated Pilo controls. In contrast, smaller spike amplitude was recorded in slices from animals given RPia. In summary, the administration of A(1) agonists reduced hippocampal excitability but not the frequency of spontaneous recurrent seizures in chronic epileptic rats, whereas A(1) receptor antagonists increased both. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Disciplina Neurofisiol, Sao Paulo, Brazil
dc.description.affiliationCtr Addict & Mental Hlth, Behav Neurobiol Lab, Toronto, ON, Canada
dc.description.affiliationUniv Toronto, Toronto Western Hosp, Div Neurosurg, Toronto, ON, Canada
dc.description.affiliationUniv Fed Sao Joao del Rei, Lab Neurociencia Expt & Computac, Sao Joao Del Rei, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Disciplina Neurol Expt, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Disciplina Neurofisiol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Disciplina Neurol Expt, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipFAPEMIG
dc.description.sponsorshipCNPq
dc.description.sponsorshipFinep ((CT-SAUDE E FNS)
dc.description.sponsorshipIDFAPESP: 2011/50680-2
dc.description.sponsorshipIDFAPESP: 2012/50950-2
dc.description.sponsorshipIDFAPESP: 2012/10764-5
dc.description.sponsorshipIDFAPEMIG: PPM 00283/14
dc.description.sponsorshipIDCNPq: 310649/2011-0
dc.description.sponsorshipIDFinep ((CT-SAUDE E FNS): 1264/13
dc.format.extent168-173
dc.identifierhttp://dx.doi.org/10.1016/j.yebeh.2016.05.036
dc.identifier.citationEpilepsy & Behavior. San Diego, v. 61, p. 168-173, 2016.
dc.identifier.doi10.1016/j.yebeh.2016.05.036
dc.identifier.issn1525-5050
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57467
dc.identifier.wosWOS:000381248000030
dc.language.isoeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofEpilepsy & Behavior
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAdenosineen
dc.subjectEpilepsyen
dc.subjectElectrophysiologyen
dc.subjectHippocampusen
dc.subjectSeizuresen
dc.subjectAnimal modelsen
dc.titleEffects of A1 receptor agonist/antagonist on spontaneous seizures in pilocarpine-induced epileptic ratsen
dc.typeinfo:eu-repo/semantics/article
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