Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients

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dc.citation.volume59
dc.contributor.authorLiverio, Harisson Oliveira
dc.contributor.authorRuiz, Luciana da Silva
dc.contributor.authorde Freitas, Roseli Santos
dc.contributor.authorNishikaku, Angela Satie [UNIFESP]
dc.contributor.authorde Souza, Ana Clara
dc.contributor.authorPaula, Claudete Rodrigues
dc.contributor.authorDomaneschi, Carina
dc.coverageSao Paulo
dc.date.accessioned2020-07-31T12:46:31Z
dc.date.available2020-07-31T12:46:31Z
dc.date.issued2017
dc.description.abstractThe aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species.en
dc.description.affiliationUniv Sao Paulo, Fac Odontol, Dept Estomatol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP, Brazil
dc.description.affiliationAdolfo Lutz Inst, Nucleo Ciencias Biomed, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1590/S1678-9946201759014
dc.identifier.citationRevista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017.
dc.identifier.doi10.1590/S1678-9946201759014
dc.identifier.fileS0036-46652017005000209.pdf
dc.identifier.issn0036-4665
dc.identifier.scieloS0036-46652017005000209
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56262
dc.identifier.wosWOS:000404610000003
dc.language.isoeng
dc.publisherInst Medicina Tropical Sao Paulo
dc.relation.ispartofRevista Do Instituto De Medicina Tropical De Sao Paulo
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCandida dubliniensisen
dc.subjectCandida albicansen
dc.subjectChildrenen
dc.subjectHIV/AIDS Phenotypic and genotypic methodsen
dc.titlePhenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patientsen
dc.typeinfo:eu-repo/semantics/article
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