Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats

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dc.contributor.authorBeltrame, Flavia Luciana [UNIFESP]
dc.contributor.authorYamauti, Caroline T.
dc.contributor.authorCaneguim, Breno Henrique [UNIFESP]
dc.contributor.authorCerri, Paulo Sergio [UNIFESP]
dc.contributor.authorMiraglia, Sandra Maria [UNIFESP]
dc.contributor.authorSasso-Cerri, Estela [UNIFESP]
dc.contributor.institutionSao Paulo State Univ UNESP
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:35:07Z
dc.date.available2018-06-15T17:35:07Z
dc.date.issued2012-10-01
dc.description.abstractCimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature.en
dc.description.affiliationSao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2006/50324-3
dc.description.sponsorshipIDFAPESP: 2006/54776-6
dc.description.sponsorshipIDFAPESP: 2007/53207-0
dc.description.sponsorshipIDFAPESP: 2010/02409-5
dc.format.extent1343-1351
dc.identifierhttp://doi.org/10.14670/HH-27.1343
dc.identifier.citationHistology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.
dc.identifier.doi10.14670/HH-27.1343
dc.identifier.issn0213-3911
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43789
dc.identifier.wosWOS:000308583700011
dc.language.isoeng
dc.publisherF Hernandez
dc.relation.ispartofHistology And Histopathology
dc.rightsAcesso restrito
dc.subjectH-2 receptor antagonisten
dc.subjectMicrovascular densityen
dc.subjectVascular atrophyen
dc.subjectCell deathen
dc.subjectSmooth muscle cellen
dc.titleCimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult ratsen
dc.typeArtigo
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