Trisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusion

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dc.citation.issue2
dc.citation.volume11
dc.contributor.authorVasques, Enio Rodrigues
dc.contributor.authorMonteiro Cunha, Jose Eduardo
dc.contributor.authorMendonca Coelho, Ana Maria
dc.contributor.authorSampietre, Sandra N.
dc.contributor.authorPatzina, Rosely Antunes
dc.contributor.authorAbdo, Emilio Elias
dc.contributor.authorNader, Helena B. [UNIFESP]
dc.contributor.authorTersariol, Ivarne L. S. [UNIFESP]
dc.contributor.authorLima, Marcelo Andrade [UNIFESP]
dc.contributor.authorGodoy, Carlos M. G. [UNIFESP]
dc.contributor.authorRodrigues, Tiago
dc.contributor.authorChaib, Eleazar
dc.contributor.authorD'Albuquerque, Luiz A. C.
dc.coverageSan Francisco
dc.date.accessioned2020-08-21T17:00:22Z
dc.date.available2020-08-21T17:00:22Z
dc.date.issued2016
dc.description.abstractBackground Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives The aims of this research are to evaluate TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods Wistar rats submitted to partial liver ischemia were divided in groups: Control: (n = 10): surgical manipulation with no liver ischemia; Saline: (n = 15): rats receiving IV saline before reperfusion; and TD: (n = 15): rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-alpha, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and after treatment with TD. Results AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline Group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.en
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Gastroenterol LIM 37, Sao Paulo, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Sci & Technol, Sao Paulo, Brazil
dc.description.affiliationFed Univ ABC, Ctr Nat & Human Sci, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Sci & Technol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0149630
dc.identifier.citationPlos One. San Francisco, v. 11, n. 2, p. -, 2016.
dc.identifier.doi10.1371/journal.pone.0149630
dc.identifier.fileWOS000371276100115.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57963
dc.identifier.wosWOS:000371276100115
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleTrisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusionen
dc.typeinfo:eu-repo/semantics/article
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