Optical anisotropy of alcian blue-stained acid glycosaminoglycans

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dc.contributor.authorMello, Maria Luiza S.
dc.contributor.authorMichelacci, Yara M.
dc.contributor.authorStockert, Juan Carlos
dc.contributor.authorVidal, Benedicto de Campos
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionAutonomous Univ Madrid
dc.date.accessioned2016-01-24T12:41:43Z
dc.date.available2016-01-24T12:41:43Z
dc.date.issued2007-01-01
dc.description.abstractOptical anisotropy as dispersion of birefringence (DB) (birefringence studied for light of different wavelengths) and linear dichroism (LD) (selective absorption of polarized Light) in stained substrates reflects their macromolecular orientation states. Birefringence interference colors of alcian blue (AB)-stained glycosamino-glycans (GAG) and glycoconjugates observed with polarization microscopy have been found to vary, although their staining characteristics under unpolarized Light are practically the same. We investigated the optical anisotropy of GAG-AB and some glycoconjugate-AB complexes used as standards, to provide a basis for interpreting results for AB-stained materials in situ. Filamentous preparations of hyaluronic acid (HA), chondroitinsulfates, proteoglycans, and a mucus sulfoglycoconjugate were studied. Anomalous DB (birefringence sign changing with the wavelength of the incident Light) was generally observed, but LD was seen only in the AB-HA complex. LD simultaneous to anomalous DB characteristics on the AB-HA complex were assumed to be caused by a maximally oriented helical conformation of the HA. for the other AB-GAG studied, the optical anisotropic characteristics were suggestive of some degree of folding of their chains into a tertiary structure. the profiles of the anomalous DB curves for the AB-stained sulfoglycoconjugate differed from those of the other materials, probably due to different organization of its dye-binding sites. (C) 2006 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationAutonomous Univ Madrid, Dept Biol, E-28049 Madrid, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent78-85
dc.identifierhttp://dx.doi.org/10.1016/j.acthis.2006.06.008
dc.identifier.citationActa Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 109, n. 1, p. 78-85, 2007.
dc.identifier.doi10.1016/j.acthis.2006.06.008
dc.identifier.issn0065-1281
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29369
dc.identifier.wosWOS:000244594300008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofActa Histochemica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectacid glycosaminoglycansen
dc.subjectalcian blueen
dc.subjectdispersion of birefringenceen
dc.subjectlinear dichroismen
dc.subjectmacromolecular orientationen
dc.subjectoptical anisotropyen
dc.titleOptical anisotropy of alcian blue-stained acid glycosaminoglycansen
dc.typeinfo:eu-repo/semantics/article
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