A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients

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dc.contributor.authorBisio, L. C.
dc.contributor.authorSilva, S. P.
dc.contributor.authorPereira, I. S.
dc.contributor.authorXavier, MAS
dc.contributor.authorVenancio, E. J.
dc.contributor.authorPuccia, R.
dc.contributor.authorSoares, CMA
dc.contributor.authorFelipe, MSS
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionNovo Nordisk Prod Farmaceut Brasil
dc.date.accessioned2016-01-24T12:38:03Z
dc.date.available2016-01-24T12:38:03Z
dc.date.issued2005-09-01
dc.description.abstractA cDNA coding for a new member of the 70-kDa heat shock proteins (HSP70) family from the dimorphic and pathogenic fungus, Paracoccidioides brasiliensis, was cloned and characterized. the cDNA-deduced sequence coded for 655 amino acid residues and showed 95% identity to a previously described P. brasiliensis hsp70 gene. Cytoplasmic and typical nuclear localization signals, which indicate induction upon stress, were identified in the deduced peptide. the complete hsp70 cDNA coding region was cloned into a pGEX 4T-3 plasmid and expressed in Escherichia coli as a glutathione-S-transferase-tagged fusion protein. the recombinant protein reacted with a rabbit polyclonal antibody against HSP70. Western immunoblot experiments demonstrated that sera from paracoccidioidomycosis patients recognized the purified recombinant protein, suggesting an immunological role for this protein in the infectious process. the antigenicity analysis of rHSP70 detected three internal peptides that could act as activators of T-cell proliferation.en
dc.description.affiliationUniv Brasilia, Mol Biol Lab, IB, BR-70910900 Brasilia, DF, Brazil
dc.description.affiliationUniv Fed Goias, Mol Biol Lab, Goiania, Go, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Ciencias Patol, Londrina, Brazil
dc.description.affiliationNovo Nordisk Prod Farmaceut Brasil, DESET ALP, Montes Claros, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent495-503
dc.identifierhttp://dx.doi.org/10.1080/13693780400029478
dc.identifier.citationMedical Mycology. Abingdon: Taylor & Francis Ltd, v. 43, n. 6, p. 495-503, 2005.
dc.identifier.doi10.1080/13693780400029478
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28456
dc.identifier.wosWOS:000233320300003
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofMedical Mycology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.subjectantigenic peptidesen
dc.subjectHSP70en
dc.subjectimmunoreactivityen
dc.subjectParacoccidioides brasiliensisen
dc.subjectparacoccidioidomycosisen
dc.subjectprotein expressionen
dc.titleA new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patientsen
dc.typeinfo:eu-repo/semantics/article
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