Selective inhibitors of digestive enzymes from Aedes aegypti larvae identified by phage display

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dc.contributor.authorSoares, Tatiane Sanches [UNIFESP]
dc.contributor.authorSoares Torquato, Ricardo Jose [UNIFESP]
dc.contributor.authorAlves Lemos, Francisco Jose
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Norte Fluminense
dc.date.accessioned2016-01-24T14:31:02Z
dc.date.available2016-01-24T14:31:02Z
dc.date.issued2013-01-01
dc.description.abstractDengue is a serious disease transmitted by the mosquito Aedes aegypti during blood meal feeding. It is estimated that the dengue virus is transmitted to millions of individuals each year in tropical and subtropical areas. Dengue control strategies have been based on controlling the vector, Ae. aegypti, using insecticide, but the emergence of resistance poses new challenges. the aim of this study was the identification of specific protease inhibitors of the digestive enzymes from Ae. aegypti larvae, which may serve as a prospective alternative biocontrol method. High affinity protein inhibitors were selected by all of the digestive serine proteases of the 4th instar larval midgut, and the specificity of these inhibitors was characterized. These inhibitors were obtained from a phage library displaying variants of HiTI, a trypsin inhibitor from Haematobia irritans, that are mutated in the reactive loop (P1 P4'). Based on the selected amino acid sequence pattern, seven HiTI inhibitor variants were cloned, expressed and purified. the results indicate that the HiTI variants named T6 (RGGAV) and T128 (WNEGL) were selected by larval trypsin-like (IC50 of 1.1 nM) and chymotrypsin-like enzymes (IC50 of 11.6 nM), respectively. the variants 123 (LLGGL) and 1149 (GGVWR) inhibited both larval chymotrypsin-like (IC50 of 4.2 nM and 29.0 nM, respectively) and elastase-like enzymes (IC50 of 1.2 nM for both). Specific inhibitors were successfully obtained for the digestive enzymes of Ae. aegypti larvae by phage display. Our data also strongly suggest the presence of elastase-like enzymes in Ae. aegypti larvae. the Hill variants T6 and T23 are good candidates for the development as a larvicide to control the vector. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Estadual Norte Fluminense, Lab Biotecnol, Rio de Janeiro, RJ, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipINCT - Entomologia Molecular
dc.description.sponsorshipIDFAPESP: 05/03514-9
dc.description.sponsorshipIDFAPESP: 09/05405-3
dc.description.sponsorshipIDCNPq: 490574/2006-8
dc.format.extent9-16
dc.identifierhttp://dx.doi.org/10.1016/j.ibmb.2012.10.007
dc.identifier.citationInsect Biochemistry and Molecular Biology. Oxford: Pergamon-Elsevier B.V., v. 43, n. 1, p. 9-16, 2013.
dc.identifier.doi10.1016/j.ibmb.2012.10.007
dc.identifier.issn0965-1748
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35802
dc.identifier.wosWOS:000313934800002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInsect Biochemistry and Molecular Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMosquitoen
dc.subjectDigestive enzymesen
dc.subjectPhage displayen
dc.subjectLarvaeen
dc.subjectAedesen
dc.titleSelective inhibitors of digestive enzymes from Aedes aegypti larvae identified by phage displayen
dc.typeinfo:eu-repo/semantics/article
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