The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: A scintigraphic study in rats

Página do item simplificado
dc.contributor.authorPrado, Maria José Brandão de Almeida [UNIFESP]
dc.contributor.authorNicastri, Ana Lucia
dc.contributor.authorCosta, P. L. A.
dc.contributor.authorRockman, T.
dc.contributor.authorTersariol, Ivarne Luis dos Santos [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorBarros, Rubens Toledo
dc.contributor.authorPrado, Euthymia Brandão de Almeida
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:23Z
dc.date.available2016-01-24T12:30:23Z
dc.date.issued1997-07-01
dc.description.abstractThe aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. the chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal distribution were established and are reported as percent of injected dose. Liver uptake of BJg was significantly higher than liver uptake of BJs (94.3 vs 81.4%) (P<0.05). This contributed to its greater removal from the intravascular compartment, and consequently lower kidney accumulation of BJg in comparison to BJs (5.7 vs 18.6%) (P<0.05). After carbohydrate oxidation, there was a decrease in hepatic accumulation of both proteins and consequently a higher renal overload. the tissue distribution of periodate-treated BJg was similar to that of native BJs: 82.7 vs 81.4% in the liver and 17.3 vs 18.6% in the kidneys. These observations indicate the important role of sugar residues of Bence Jones proteins for their recognition by specific membrane receptors, which leads to differential tissue accumulation and possible toxicity.en
dc.description.affiliationUNIV São Paulo,FAC MED,LAB FISIOPATOL RENAL,BR-01246903 São Paulo,SP,BRAZIL
dc.description.affiliationUNIV São Paulo,CTR MED NUCL,BR-05403010 São Paulo,SP,BRAZIL
dc.description.affiliationUNIV São Paulo,HOSP CLIN,SERV RADISISOTOPOS,BR-05403000 São Paulo,SP,BRAZIL
dc.description.affiliationUniversidade Federal de São Paulo,MOL BIOL LAB,BR-04024900 São Paulo,SP,BRAZIL
dc.description.affiliationUnifespUniversidade Federal de São Paulo,MOL BIOL LAB,BR-04024900 São Paulo,SP,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent865-872
dc.identifierhttps://dx.doi.org/10.1590/S0100-879X1997000700008
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 30, n. 7, p. 865-872, 1997.
dc.identifier.doi10.1590/S0100-879X1997000700008
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1997000700008
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/25751
dc.identifier.wosWOS:A1997XN66100008
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBence Jones proteinsen
dc.subjectScintigraphic studyen
dc.subjectMultiple myelomaen
dc.subjectNephrotoxicityen
dc.titleThe renal and hepatic distribution of Bence Jones proteins depends on glycosylation: A scintigraphic study in ratsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos