Venous Shunt Versus Venous Ligation for Vascular Damage Control: The Immunohistochemical Evidence

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dc.citation.volume41
dc.contributor.authorde Oliveira Goes Junior, Adenauer Marinho [UNIFESP]
dc.contributor.authorVieira Abib, Simone de Campos [UNIFESP]
dc.contributor.authorde Seixas Alves, Maria Teresa [UNIFESP]
dc.contributor.authorVenerando da Silva Ferreira, Paulo Sergio [UNIFESP]
dc.contributor.authorde Andrade, Mariseth Carvalho [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-07-13T11:53:22Z
dc.date.available2020-07-13T11:53:22Z
dc.date.issued2017
dc.description.abstractBackground: To evaluate the expression of immunohistochemical markers of tissue ischemia (iNOS, eNOS, and HSP70) in a vascular damage control experimental model to determine if a venous temporary vascular shunt insertion leads to a better limb perfusion when compared with the ligature of the injured vein. Methods: Experimental study in male Sus Scrofa weighting 40 Kg. Animals were distributed into 5 groups: group 1 animals were submitted to right external iliac artery (EIA) shunting and right external iliac vein (EIV) ligationen
dc.description.abstractgroup 2 animals were submitted to right EIA shunting and right EIV shuntingen
dc.description.abstractgroup 3 animals were submitted to right EIV ligationen
dc.description.abstractgroup 4 animals were submitted to right EIV shuntingen
dc.description.abstractgroup 5 animals were not submitted to vascular shunting or venous ligation. Transonic Systems flowmeters were used to measure vascular flow on right and left external iliac vessels, and i-STAT (Abbot) portable blood analyzer was used for EIVs blood biochemical analysis. An initial baseline register of invasive arterial pressure, iliac vessels flow, and venous blood analysis was performed. Arterial pressure and iliac vessels flow were taken immediately after right iliac vessels shunting or ligation. Then, hemorrhagic shock was induced by continuous 20 mL/min blood withdraw from the external right jugular vein whereas arterial blood pressure and iliac vessels flow registers were taken every 10 min, and blood samples from EIVs were obtained every 30 min until the vascular flow through right EIA (or through the shunt inserted into the right EIV for group 4 animals) became inexistent or until the animal's death. After the end of the experiments, bilateral hind limb's biopsies were obtained for immunohistochemical analysis. Using image editing and analysis software, the expression of iNOS, eNOS, and HSP70 (3 well-known ischemic associated immunohistochemical markers) was assessed. The mean expression of each marker in the right hind limb was compared between groups. For statistical analysis, Microsoft Office Excel 2007 and BioEstat 5.0 (2007) were used. Results: Immunohistochemical analysis showed no difference regarding the iNOS expressionen
dc.description.abstractnevertheless, both eNOS and HSP70 expression were statistically more intense (P < 0.05) on group 1 (eNOS = 1.32en
dc.description.abstractHSP70 = 15.05) than on group 2 (eNOS = 0.018en
dc.description.abstractHSP70 = 8.56). Conclusions: The higher expression of eNOS and HSP70 in the right hind limbs of group 1 animals (arterial shunt and venous ligature) than group 2 animals (arterial shunt and venous shunt) suggests that venous ligation is associated with more intense ischemic histological findings than venous shunting.en
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Surg, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Surg, Sao Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2020-07-13T11:53:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2017en
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation
dc.description.sponsorshipIDFAPESP: 2010/17624-9
dc.format.extent214-224
dc.identifierhttp://dx.doi.org/10.1016/j.avsg.2016.10.031
dc.identifier.citationAnnals Of Vascular Surgery. New York, v. 41, p. 214-224, 2017.
dc.identifier.doi10.1016/j.avsg.2016.10.031
dc.identifier.fileWOS000402927300047.pdf
dc.identifier.issn0890-5096
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54567
dc.identifier.wosWOS:000402642100042
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofAnnals Of Vascular Surgery
dc.relation.ispartof41st Annual Meeting of the Vascular-and-Endovascular-Surgery-Society
dc.rightsAcesso restrito
dc.titleVenous Shunt Versus Venous Ligation for Vascular Damage Control: The Immunohistochemical Evidenceen
dc.typeArtigo
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