Salivary Alterations in Rats with Experimental Chronic Kidney Disease

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dc.citation.issue2
dc.citation.volume11
dc.contributor.authorRomero, Ana Carolina
dc.contributor.authorBergamaschi, Cassia Toledo [UNIFESP]
dc.contributor.authorde Souza, Douglas Nesadal
dc.contributor.authorNogueira, Fernando Neves
dc.coverageSan Francisco
dc.date.accessioned2020-08-21T17:00:24Z
dc.date.available2020-08-21T17:00:24Z
dc.date.issued2016
dc.description.abstractObjective This study aimed to analyze changes in saliva composition and salivary secretion process of rats with chronic kidney disease induced by 5/6 nephrectomy to set the foundation for salivary studies related to CKD. Methods CKD was induced in Wistar rats via 5/6 nephrectomy. Blood and saliva samples were collected from Control, Sham and CKD groups at 8 and 12 weeks after the surgery. Salivation was stimulated via intraperitoneal injections of pilocarpine (1.0 mg/Kg body weight) or isoproterenol (5.0 mg/Kg body weight). Saliva was collected and immediately stored at -80 degrees C until analysis. The salivary flow rate, total protein, amylase and peroxidase activities, and urea concentrations were measured. The blood urea nitrogen (BUN) and serum creatinine concentrations were also evaluated. Results Increases in BUN and serum creatinine concentrations were observed in the CKD groups. Amylase activity was significantly reduced in response to both stimuli in the CKD groups at 8 weeks and increased in the CKD groups at 12 weeks in response to isoproterenol stimulus. The peroxidase activities of the CKD groups were significantly reduced in response to isoproterenol stimulation and were increased at 12 weeks in response to pilocarpine stimulation. Salivary urea was significantly increased in the CKD groups at 8 weeks in response to the isoproterenol stimuli and at 12 weeks in response to both salivary agonists. Conclusions The pattern of alterations observed in this experimental model is similar to those observed in patients and clearly demonstrates the viability of 5/6 nephrectomy as an experimental model in future studies to understand the alterations in salivary compositions and in salivary glands that are elicited by CKD.en
dc.description.affiliationUniv Sao Paulo, Dept Biomat & Biol Oral, Fac Odontol, Sao Paulo, Brazil
dc.description.affiliationEscola Paulista Med UNIFESP, Dept Fisiol, Sao Paulo, Brazil
dc.description.affiliationUnifespEscola Paulista Med UNIFESP, Dept Fisiol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP (the Research Council for the State of Sao Paulo)
dc.description.sponsorshipIDFAPESP: 2013-18609-1
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0148742
dc.identifier.citationPlos One. San Francisco, v. 11, n. 2, p. -, 2016.
dc.identifier.doi10.1371/journal.pone.0148742
dc.identifier.fileWOS000370038400073.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57980
dc.identifier.wosWOS:000370038400073
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSalivary Alterations in Rats with Experimental Chronic Kidney Diseaseen
dc.typeinfo:eu-repo/semantics/article
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