Gene expression in the salivary complexes from Haementeria depressa leech through the generation of expressed sequence tags

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dc.contributor.authorFaria, F.
dc.contributor.authorJunqueira-de-Azevedo, I. D.
dc.contributor.authorHo, P. L.
dc.contributor.authorSampaio, M. U.
dc.contributor.authorChudzinski-Tavassi, A. M.
dc.contributor.institutionInst Bunantan
dc.contributor.institutionInst Buntantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:47Z
dc.date.available2016-01-24T12:37:47Z
dc.date.issued2005-04-11
dc.description.abstractA survey of the transcriptional profile of Haementeria depressa Ringuelet, 1972 (Annelida, Hirudinea) salivary complexes was produced through expressed sequence tag (EST). Sequences from 898 independent clones were assembled in 555 clusters, representing the transcript profile of this tissue. the repertoire of possible proteins involved in feeding and host interaction processes of the leech corresponded to 10.6% of all identified transcripts (67 clusters), being the carbonic anhydrases (30%), several coagulation inhibitors (25%) and hemerythrin-like molecules (19%), the major components. Among the 387 clusters matching cellular proteins, the majority represents molecules involved in gene and protein expression, reflecting a high specialization of this tissue for protein synthesis. Our H. depressa dbEST was also compared to those from other blood-feeding organisms, providing evidences that among the secreted proteins, the coagulation inhibitors present a profile very characteristic of this animal class. Published by Elsevier B.V.en
dc.description.affiliationInst Bunantan, Lab Bioquim & Biofis, BR-05503900 São Paulo, Brazil
dc.description.affiliationInst Buntantan, Ctr Biotecnol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biociencias, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent173-185
dc.identifierhttp://dx.doi.org/10.1016/j.gene.2004.12.022
dc.identifier.citationGene. Amsterdam: Elsevier B.V., v. 349, p. 173-185, 2005.
dc.identifier.doi10.1016/j.gene.2004.12.022
dc.identifier.issn0378-1119
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28248
dc.identifier.wosWOS:000228757600017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofGene
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectleechesen
dc.subjecttranscriptomeen
dc.subjectexpressed sequence tagen
dc.subjectblood-feedingen
dc.subjectcoagulation inhibitorsen
dc.titleGene expression in the salivary complexes from Haementeria depressa leech through the generation of expressed sequence tagsen
dc.typeinfo:eu-repo/semantics/article
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