Effects of Repeated Stress on Distal Airway Inflammation, Remodeling and Mechanics in an Animal Model of Chronic Airway Inflammation

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dc.contributor.authorLeick, Edna Aparecida
dc.contributor.authorReis, Fabiana Gomes
dc.contributor.authorHonorio-Neves, Flavia Alves
dc.contributor.authorAlmeida-Reis, Rafael
dc.contributor.authorPrado, Carla Máximo [UNIFESP]
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorTiberio, Iolanda de Fátima Lopes Calvo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:46Z
dc.date.available2016-01-24T14:17:46Z
dc.date.issued2012-01-01
dc.description.abstractBackground/Aims: Epidemiological studies suggest that stress has an impact on asthmatic exacerbations. We evaluated if repeated stress, induced by forced swimming, modulates lung mechanics, distal airway inflammation and extracellular matrix remodeling in guinea pigs with chronic allergic inflammation. Methods: Guinea pigs were submitted to 7 ovalbumin or saline aerosols (1-5 mg/ml during 4 weeks; OVA and SAL groups). Twenty-four hours after the 4th inhalation, guinea pigs were submitted to the stress protocol 5 times a week during 2 weeks (SAL-S and OVA-S groups). Seventy-two hours after the 7th inhalation, guinea pigs were anesthetized and mechanically ventilated. Resistance and elastance of the respiratory system were obtained at baseline and after ovalbumin challenge. Lungs were removed, and inflammatory and extracellular matrix remodeling of distal airways was assessed by morphometry. Adrenals were removed and weighed. Results: the relative adrenal weight was greater in stressed guinea pigs compared to non-stressed animals (p < 0.001). Repeated stress increased the percent elastance of the respiratory system after antigen challenge and eosinophils and lymphocytes in the OVA-S compared to the OVA group (p < 0.001, p = 0.003 and p < 0.001). Neither collagen nor elastic fiber contents were modified by stress in sensitized animals. Conclusions: in this animal model, repeated stress amplified bronchoconstriction and inflammatory response in distal airways without interfering with extracellular matrix remodeling. Copyright (C) 2011 S. Karger AG, Baselen
dc.description.affiliationUniv São Paulo, Dept Med, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.sourceWeb of Science
dc.format.extent1-9
dc.identifierhttps://dx.doi.org/10.1159/000324686
dc.identifier.citationNeuroimmunomodulation. Basel: Karger, v. 19, n. 1, p. 1-9, 2012.
dc.identifier.doi10.1159/000324686
dc.identifier.issn1021-7401
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34490
dc.identifier.wosWOS:000296750800001
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofNeuroimmunomodulation
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectStressen
dc.subjectExperimental asthma modelen
dc.subjectExtracellular matrix remodelingen
dc.subjectEosinophilsen
dc.titleEffects of Repeated Stress on Distal Airway Inflammation, Remodeling and Mechanics in an Animal Model of Chronic Airway Inflammationen
dc.typeinfo:eu-repo/semantics/article
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