PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO SHARK CARTILAGE PROTEOGLYCAN
| dc.contributor.author | Alves, MLM | |
| dc.contributor.author | Straus, Anita Hilda [UNIFESP] | |
| dc.contributor.author | Takahashi, Helio Kiyoshi [UNIFESP] | |
| dc.contributor.author | Michelacci, Yara Maria [UNIFESP] | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2018-06-15T14:28:09Z | |
| dc.date.available | 2018-06-15T14:28:09Z | |
| dc.date.issued | 1994-09-01 | |
| dc.description.abstract | 1. Two proteoglycans, PG1 and PG2, have been isolated from shark cartilage. Both are highly polydisperse and large (molecular mass: 1-10 X 10(6) Daltons) and contain chondroitin sulfate and keratan sulfate side chains, but PG2 is somewhat smaller than PG1 and contains less keratan sulfate.2. Monoclonal antibodies were raised against PG1. Many antibodies were obtained and one of them, MST1, was subcloned and further characterized. This monoclonal antibody reacts with PG1 and PG2 from shark cartilage and also with aggrecan from bovine trachea cartilage. Chondroitinase AC-treated proteoglycans react with MST1, indicating that the antibody does not recognize chondroitin sulfate. MST1 also recognizes aggrecan from human cartilage and a proteoglycan from bovine brain (neurocan) but it does not recognize proteoglycans from rat Walker tumor, fetal calf muscle and decorin from human myoma.3. Using MST1 we were able to demonstrate that both PG1 and PG2 aggregate with hyaluronic acid. | en |
| dc.description.affiliation | ESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04044020 SAO PAULO,BRAZIL | |
| dc.description.affiliationUnifesp | ESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04044020 SAO PAULO,BRAZIL | |
| dc.description.source | Web of Science | |
| dc.format.extent | 2103-2108 | |
| dc.identifier.citation | Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 9, p. 2103-2108, 1994. | |
| dc.identifier.issn | 0100-879X | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/43074 | |
| dc.identifier.wos | WOS:A1994PG03900003 | |
| dc.language.iso | eng | |
| dc.publisher | Assoc Bras Divulg Cientifica | |
| dc.relation.ispartof | Brazilian Journal Of Medical And Biological Research | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.subject | SHARK CARTILAGE PROTEOGLYCANS | en |
| dc.subject | MONOCLONAL ANTIBODIES | en |
| dc.title | PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO SHARK CARTILAGE PROTEOGLYCAN | en |
| dc.type | info:eu-repo/semantics/article |