Glial cells modulate heparan sulfate proteoglycan (HSPG) expression by neuronal precursors during early postnatal cerebellar development

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dc.contributor.authorAraujo, Ana Paula B. [UNIFESP]
dc.contributor.authorRibeiro, Maria Emilia de Oliveira Brenha [UNIFESP]
dc.contributor.authorRicci, Ritchelli [UNIFESP]
dc.contributor.authorTorquato, Ricardo José Soares [UNIFESP]
dc.contributor.authorToma, Leny [UNIFESP]
dc.contributor.authorPorcionatto, Marimélia Aparecida [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInvestiga Inst Pesquisa
dc.date.accessioned2016-01-24T14:05:38Z
dc.date.available2016-01-24T14:05:38Z
dc.date.issued2010-11-01
dc.description.abstractCerebellum controls motor coordination, balance, eye movement, and has been implicated in memory and addiction. As in other parts of the CNS, correct embryonic and postnatal development of the cerebellum is crucial for adequate performance in the adult. Cellular and molecular defects during cerebellar development can lead to severe phenotypes, such as ataxias and tumors. Knowing how the correct development occurs can shed light into the mechanisms of disease. Heparan sulfate proteoglycans are complex molecules present in every higher eukaryotic cells and changes in their level of expression as well as in their structure lead to drastic functional alterations. This work aimed to investigate changes in heparan sulfate proteoglycans expression during cerebellar development that could unveil control mechanisms. Using real time RT-PCR we evaluated the expression of syndecans, glypicans and modifying enzymes by isolated cerebellar granule cell precursors, and studied the influence of soluble glial factors on the expression of those genes. We evaluated the possible involvement of Runx transcription factors in the response of granule cell precursors to glial factors. Our data show for the first time that cerebellar granule cell precursors express members of the Runx family and that the expression of those genes can also be controlled by glial factors. Our results also show that the expression of all genes studied vary during postnatal development and treatment of precursors with glial factors indicate that the expression of heparan sulfate proteoglycan genes as well as genes encoding heparan sulfate modifying enzymes can be modulated by the microenvironment, reflecting the intricate relations between neuron and glia. (C) 2010 ISDN. Published by Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationInvestiga Inst Pesquisa, BR-13084791 Campinas, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipInvestiga Institutos de Pesquisa
dc.format.extent611-620
dc.identifierhttp://dx.doi.org/10.1016/j.ijdevneu.2010.07.228
dc.identifier.citationInternational Journal of Developmental Neuroscience. Oxford: Pergamon-Elsevier B.V., v. 28, n. 7, p. 611-620, 2010.
dc.identifier.doi10.1016/j.ijdevneu.2010.07.228
dc.identifier.issn0736-5748
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33040
dc.identifier.wosWOS:000282905900008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Developmental Neuroscience
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCerebellumen
dc.subjectGlypicanen
dc.subjectSyndecanen
dc.subjectRunxen
dc.subjectGranule cell precursoren
dc.titleGlial cells modulate heparan sulfate proteoglycan (HSPG) expression by neuronal precursors during early postnatal cerebellar developmenten
dc.typeinfo:eu-repo/semantics/article
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