Eicosapentaenoic acid and docosahexaenoic acid exert anti-inflammatory and antinociceptive effects in rodents at low doses

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dc.contributor.authorPereira Nobre, Maria Elizabeth [UNIFESP]
dc.contributor.authorCorreia, Alyne Oliveira
dc.contributor.authorBorges, Marilia de Brito
dc.contributor.authorAraujo Sampaio, Thayga Maria
dc.contributor.authorChakraborty, Solon Arcoverde
dc.contributor.authorGoncalves, Danilo de Oliveira
dc.contributor.authorCastro Brito, Gerly Anne de
dc.contributor.authorAlmeida Moreira Leal, Luzia Kalyne
dc.contributor.authorBezerra Felipe, Cicero Francisco
dc.contributor.authorLucetti, Daniel Luna
dc.contributor.authorArida, Ricardo Mario [UNIFESP]
dc.contributor.authorBarros Viana, Glauce Socorro de
dc.contributor.institutionFac Med Estado Juazeiro do Norte FMJ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFed Univ Ceara UFC
dc.date.accessioned2016-01-24T14:31:43Z
dc.date.available2016-01-24T14:31:43Z
dc.date.issued2013-05-01
dc.description.abstractIn the present study, we evaluated omega-3 polyunsaturated fatty acid (PUPA) (consisting of 20:5n-3 and 22:6n-3) properties on inflammation and nociception. Among the in vivo tests, writhing, formalin, and hot plate tests were conducted in mice, and carrageenan-induced paw edema, peritonitis, and Hargreaves tests were performed in rats. Following the carrageenan-induced edema, immunohistochemistry for tumor necrosis factor-alpha (TNF-alpha) was also carried out. We found that omega-3 PUPA treatment significantly decreased acetic acid-induced abdominal contortions as well as the first and second phases of the formalin test, which were reversed by naloxone. the carrageenan-induced rat paw edema was significantly reduced, along with neutrophil migration to the peritoneal cavity in the omega-3 PUPA treatment. in addition, there was a decrease in TNF-a immunostained cells in the inflamed paw with the omega-3 treatment compared with no omega-3. Withdrawal threshold in response to the thermal stimulation was significantly increased by the omega-3 treatment in the Hargreaves and hot plate tests. the in vitro studies (myeloperoxidase, lactate dehydrogenase, MTT cell viability and lipid peroxidation assays) were performed in human neutrophils. These studies showed that omega-3 treatment significantly decreased myeloperoxidase release, presented no cytotoxicity, and did not alter lipid peroxidation. Our study suggests that omega-3 PUPA anti-inflammatory and antinociceptive actions may involve inhibition of cyclooxygenases and microglial activation, leading to a reduced release of proinflammatory cytokines such as TNF-alpha, among other factors. the omega-3 PUFAs are potential candidates used alone or in combination with conventional nonsteroidal anti-inflammatory drugs, for the treatment of diseases where inflammation plays an important role. (C) 2013 Elsevier Inc. All rights reserved.en
dc.description.affiliationFac Med Estado Juazeiro do Norte FMJ, Juazeiro Do Norte, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, São Paulo, Brazil
dc.description.affiliationFed Univ Ceara UFC, Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFoundation for Research and Development of the State of Ceara (FUNCAP)
dc.format.extent422-433
dc.identifierhttp://dx.doi.org/10.1016/j.nutres.2013.02.011
dc.identifier.citationNutrition Research. Oxford: Pergamon-Elsevier B.V., v. 33, n. 5, p. 422-433, 2013.
dc.identifier.doi10.1016/j.nutres.2013.02.011
dc.identifier.issn0271-5317
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36298
dc.identifier.wosWOS:000319856500010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNutrition Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectOmega-3 polyunsaturated fatty acidsen
dc.subjectAnti-inflammatory and anti-nociceptive actionsen
dc.subjectCytotoxicityen
dc.subjectLipid peroxidationen
dc.subjectAntioxidant effectsen
dc.subjectNeutrophilsen
dc.titleEicosapentaenoic acid and docosahexaenoic acid exert anti-inflammatory and antinociceptive effects in rodents at low dosesen
dc.typeinfo:eu-repo/semantics/article
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