Structural features and anticoagulant activities of a novel natural low molecular weight heparin from the shrimp Penaeus brasiliensis

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dc.contributor.authorDietrich, C. P.
dc.contributor.authorPaiva, J. F.
dc.contributor.authorCastro, RAB
dc.contributor.authorChavante, S. F.
dc.contributor.authorJeske, W.
dc.contributor.authorFareed, J.
dc.contributor.authorGorin, PAJ
dc.contributor.authorMendes, A.
dc.contributor.authorNader, H. B.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Rio Grande Norte
dc.contributor.institutionLoyola Med Sch
dc.contributor.institutionUniv Fed Parana
dc.date.accessioned2016-01-24T12:30:52Z
dc.date.available2016-01-24T12:30:52Z
dc.date.issued1999-08-05
dc.description.abstractA natural low molecular weight heparin (8.5 kDa), with an anticoagulant activity of 95 IU/mg by the USP assay, was isolated from the shrimp Penaeus brasiliensis. the crustacean heparin was susceptible to both heparinase and heparitinase II from Flavobacterium heparinum forming tri- and di-sulfated disaccharides as the mammalian heparins. C-13 and H-1 NMR spectroscopy revealed that the shrimp heparin was enriched in both glucuronic and non-sulfated iduronic acid residues. the in vitro anticlotting activities in different steps of the coagulation cascade have shown that its anticoagulant action is mainly exerted through the inhibition of factor Xa and heparin cofactor II-mediated inhibition of thrombin. the shrimp heparin has also a potent in vivo antithrombotic activity comparable to the mammalian low molecular weight heparins. (C) 1999 Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande Norte, Dept Bioquim, Natal, RN, Brazil
dc.description.affiliationLoyola Med Sch, Dept Pathol, Maywood, IL USA
dc.description.affiliationUniv Fed Parana, Dept Bioquim, BR-80060000 Curitiba, Parana, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent273-283
dc.identifierhttp://dx.doi.org/10.1016/S0304-4165(99)00087-2
dc.identifier.citationBiochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1428, n. 2-3, p. 273-283, 1999.
dc.identifier.doi10.1016/S0304-4165(99)00087-2
dc.identifier.issn0304-4165
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26128
dc.identifier.wosWOS:000082142200016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica Et Biophysica Acta-general Subjects
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectshrimp heparin structureen
dc.subjectshrimp heparin NMRen
dc.subjectinvertebrate heparinen
dc.subjectnatural low molecular weight shrimp heparinen
dc.subjectshrimp heparinen
dc.subjectanticlotting activityen
dc.subjectantithrombotic activityen
dc.subjectCrustacean heparinen
dc.titleStructural features and anticoagulant activities of a novel natural low molecular weight heparin from the shrimp Penaeus brasiliensisen
dc.typeinfo:eu-repo/semantics/article
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