Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle

dc.contributor.authorDossin, Fernando de M.
dc.contributor.authorDufour, Alexandre
dc.contributor.authorDusch, Elodie
dc.contributor.authorSiqueira-Neto, Jair L.
dc.contributor.authorMoraes, Carolina B. [UNIFESP]
dc.contributor.authorYang, Gyong Seon
dc.contributor.authorCano, Maria Isabel
dc.contributor.authorGenovesio, Auguste
dc.contributor.authorFreitas-Junior, Lucio H.
dc.contributor.institutionInst Pasteur Korea
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Paulista
dc.date.accessioned2016-01-24T13:51:28Z
dc.date.available2016-01-24T13:51:28Z
dc.date.issued2008-06-11
dc.description.abstractParasite virulence genes are usually associated with telomeres. the clustering of the telomeres, together with their particular spatial distribution in the nucleus of human parasites such as Plasmodium falciparum and Trypanosoma brucei, has been suggested to play a role in facilitating ectopic recombination and in the emergence of new antigenic variants. Leishmania parasites, as well as other trypanosomes, have unusual gene expression characteristics, such as polycistronic and constitutive transcription of protein-coding genes. Leishmania subtelomeric regions are even more unique because unlike these regions in other trypanosomes they are devoid of virulence genes. Given these peculiarities of Leishmania, we sought to investigate how telomeres are organized in the nucleus of Leishmania major parasites at both the human and insect stages of their life cycle. We developed a new automated and precise method for identifying telomere position in the three-dimensional space of the nucleus, and we found that the telomeres are organized in clusters present in similar numbers in both the human and insect stages. While the number of clusters remained the same, their distribution differed between the two stages. the telomeric clusters were found more concentrated near the center of the nucleus in the human stage than in the insect stage suggesting reorganization during the parasite's differentiation process between the two hosts. These data provide the first 3D analysis of Leishmania telomere organization. the possible biological implications of these findings are discussed.en
dc.description.affiliationInst Pasteur Korea, Syst Biol Pathogens Grp, Seoul, South Korea
dc.description.affiliationInst Pasteur Korea, Image Mining Grp, Seoul, South Korea
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol, Imunol Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biocien, Dept Genet, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol, Imunol Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipInstitut Pasteur Korea
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0002313
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 3, n. 6, 6 p., 2008.
dc.identifier.doi10.1371/journal.pone.0002313
dc.identifier.fileWOS000263248800003.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30728
dc.identifier.wosWOS:000263248800003
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAutomated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycleen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000263248800003.pdf
Tamanho:
480.98 KB
Formato:
Adobe Portable Document Format
Descrição:
Coleções