POSITIVE IDENTIFICATION OF ENTEROCYTES BY KERATIN ANTIBODY STAINING OF SLOUGHED INTESTINAL TISSUE IN SEVERE GVHD

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dc.contributor.authorSilva, MRR [UNIFESP]
dc.contributor.authorHenne, K.
dc.contributor.authorSale, G. E.
dc.contributor.institutionFRED HUTCHINSON CANC RES CTR
dc.contributor.institutionUNIV WASHINGTON
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T14:04:32Z
dc.date.available2018-06-15T14:04:32Z
dc.date.issued1993-07-01
dc.description.abstractNine marrow allograft recipients who developed GVHD of the gastrointestinal tract accompanied by the passage of ropey necrotic material per rectum were studied. The material, resembling necrotic intestinal mucosa, was evaluated for the presence of epithelial cells using monoclonal antibodies for keratin and macrophages. Two keratins (AE1/AE3 and 34betaE12) were detected within celts in all cases while macrophages were found in all but one case. In three cases, some cells were positive for both antibodies suggesting the presence of phagocytosed keratin in macrophage cytoplasm. Search for organisms with Gram and methenamine silver stains showed bacteria in six cases and fungus in one. Some cases of severe GVHD will be associated with the passage of ropey tan material grossly resembling sloughed mucosal tissue, but microscopically consisting largely of fibrin clots. The presence of free intestinal epithelial cells in this material is confirmed by the present study.en
dc.description.affiliationFRED HUTCHINSON CANC RES CTR,DIV CLIN,SEATTLE,WA 98104
dc.description.affiliationUNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195
dc.description.affiliationESCOLA PAULISTA MED,DEPT ANAT PATHOL,BR-04023 SAO PAULO,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT ANAT PATHOL,BR-04023 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent35-36
dc.identifier.citationBone Marrow Transplantation. Basingstoke: Stockton Press, v. 12, n. 1, p. 35-36, 1993.
dc.identifier.issn0268-3369
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/42868
dc.identifier.wosWOS:A1993LP07700006
dc.language.isoeng
dc.publisherStockton Press
dc.relation.ispartofBone Marrow Transplantation
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titlePOSITIVE IDENTIFICATION OF ENTEROCYTES BY KERATIN ANTIBODY STAINING OF SLOUGHED INTESTINAL TISSUE IN SEVERE GVHDen
dc.typeinfo:eu-repo/semantics/article
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