In vitro cytotoxicity of chemical preservatives on human fibroblast cells

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dc.citation.issue1
dc.citation.volume54
dc.contributor.authorSpindolau, Daniel Gonsales [UNIFESP]
dc.contributor.authorHinsberger, Andre
dc.contributor.authorde Souza Antunes, Valeria Maria
dc.contributor.authorGomes Michelin, Luis Felipe
dc.contributor.authorBincoletto, Claudia [UNIFESP]
dc.contributor.authorOliveira, Carlos Rocha [UNIFESP]
dc.coverageSao Paulo
dc.date.accessioned2020-07-08T13:09:56Z
dc.date.available2020-07-08T13:09:56Z
dc.date.issued2018
dc.description.abstractPreservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD 1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MIT assay and propidium iodide staining (IMU > methylparaben > sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors.en
dc.description.affiliationUniv Anhembi Morumbi, Escola Ciencias Saude, Grp Fitocomplexos & Sinalizacao Celular, Sao Paulo, SP, Brazil
dc.description.affiliationInst Osmol & Oleos Essenciais, Monte Verde, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCAPES
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1590/s2175-97902018000100031
dc.identifier.citationBrazilian Journal Of Pharmaceutical Sciences. Sao Paulo, v. 54, n. 1, p. -, 2018.
dc.identifier.doi10.1590/s2175-97902018000100031
dc.identifier.fileS1984-82502018000100603.pdf
dc.identifier.issn1984-8250
dc.identifier.scieloS1984-82502018000100603
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54312
dc.identifier.wosWOS:000432451200001
dc.language.isoeng
dc.publisherUniv Sao Paulo, Conjunto Quimicas
dc.relation.ispartofBrazilian Journal Of Pharmaceutical Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPreservativesen
dc.subjectPharmaceutical/chemistryen
dc.subjectFibroblasts/cytotoxicityen
dc.subjectCell death/drag effectsen
dc.subjectCosmetics/additivesen
dc.titleIn vitro cytotoxicity of chemical preservatives on human fibroblast cellsen
dc.typeinfo:eu-repo/semantics/article
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