Revealing the Lytic Mechanism of the Antimicrobial Peptide Gomesin by Observing Giant Unilamellar Vesicles

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dc.contributor.authorDomingues, Tatiana Moreira [UNIFESP]
dc.contributor.authorRiske, Karin A. [UNIFESP]
dc.contributor.authorMiranda, Antonio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:12Z
dc.date.available2016-01-24T14:05:12Z
dc.date.issued2010-07-06
dc.description.abstractGomesin (Gm) is a potent cationic antimicrobial peptide from a Brazilian spider. Here we use optical and fluorescence microscopy to study the interaction of Gm, its low active linear analogue, [Ser(2.6,11,15)]-Gm (GmL), and a fluorescent labeled analogue. Gm-Rh, with giant unilamellar vesicles (GUVs) composed of mixtures of the neutral lipid palmitoyloleoyl phosphatidylcholine (POPC) with the negatively charged lipid palmitoyloleoyl phosphatidylglycerol (POPG) or cholesterol, so as to mimic bacterial and mammalian cell membranes, respectively. We observed the effect of injecting a peptide solution with a micropipet close to GUVs. As a result of peptide-lipid interaction, GUVs burst suddenly. Stable pores, which result in leaky vesicles, were not observed. Fluorescence microscopy of Gm-Rh injected on GUVs confirmed the high peptide/lipid affinity. These facts lead us to suggest that Gm and GmL disrupt the membrane via the carpet model. in order to quantify the lytic activity of both peptides against different membrane composition, a solution of GUVs was diluted in increasing concentration of peptides and the fraction of burst GM's was measured as a function of time. the lytic activity of both peptides was enhanced by the presence of POPG and decreased upon addition of cholesterol, GmL exhibited lower lytic activity as compared to Gm, but this difference vanished at high POPG molar fraction.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFADA/UNIFESP
dc.description.sponsorshipinctFCx
dc.format.extent11077-11084
dc.identifierhttp://dx.doi.org/10.1021/la100662a
dc.identifier.citationLangmuir. Washington: Amer Chemical Soc, v. 26, n. 13, p. 11077-11084, 2010.
dc.identifier.doi10.1021/la100662a
dc.identifier.issn0743-7463
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32720
dc.identifier.wosWOS:000279239900091
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofLangmuir
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleRevealing the Lytic Mechanism of the Antimicrobial Peptide Gomesin by Observing Giant Unilamellar Vesiclesen
dc.typeinfo:eu-repo/semantics/article
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