Hyaluronidase Alters the Lipid Profile of Cumulus Cells as Detected by MALDI-TOF MS and Multivariate Analysis

Página do item simplificado
dc.contributor.authorMontani, Daniela Antunes [UNIFESP]
dc.contributor.authorRegiani, Thais [UNIFESP]
dc.contributor.authorVictorino, Amanda Begati [UNIFESP]
dc.contributor.authorCamillo, Jacqueline [UNIFESP]
dc.contributor.authorPilau, Eduardo Jorge
dc.contributor.authorGozzo, Fabio Cesar
dc.contributor.authorZylbersztejn, Daniel Suslik [UNIFESP]
dc.contributor.authorFerreira, Christina Ramires
dc.contributor.authorLo Turco, Edson Guimaraes [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2016-01-24T14:37:47Z
dc.date.available2016-01-24T14:37:47Z
dc.date.issued2014-09-01
dc.description.abstractThis research aimed to study the changes in lipid composition in cumulus cells using hyaluronidase according to the intracytoplasmic sperm injection protocol commonly used in human reproduction clinics. the lipid extraction was performed by the Blight-Dyer protocol and the lipid profiles were obtained by MALDI-TOF MS in positive and negative modes. the mass spectra data were processed with MassLynx and the statistical analysis was performed using MetaboAnalyst 2.0. Fifteen ions were selected for each mode as potential markers for differences between the groups. These ions were identified in the human metabolome database as phosphatidylserine with and without treatment, phosphatidylethanolamine in the after treatment group and phosphatidylinositol in the before treatment group, which are lipids that may be involved in cell apoptosis and signaling. We concluded that MALDI-TOF MS coupled with multivariate analysis can be utilized as a strategy to obtain and study the lipid profiles of cumulus cells and as a tool to study the metabolic state of cumulus cells.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2010/14,732-5
dc.format.extent957-962
dc.identifierhttp://dx.doi.org/10.1007/s11745-014-3931-6
dc.identifier.citationLipids. Heidelberg: Springer Heidelberg, v. 49, n. 9, p. 957-962, 2014.
dc.identifier.doi10.1007/s11745-014-3931-6
dc.identifier.issn0024-4201
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38144
dc.identifier.wosWOS:000341085800009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLipids
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectLipidsen
dc.subjectLipid profileen
dc.subjectCumulus cellsen
dc.subjectHyaluronidaseen
dc.subjectMALDI-TOF MSen
dc.subjectMultivariate analysisen
dc.titleHyaluronidase Alters the Lipid Profile of Cumulus Cells as Detected by MALDI-TOF MS and Multivariate Analysisen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos