Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

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dc.contributor.authorPerez, Rogerio Renato
dc.contributor.authorGoldenberg, Alberto [UNIFESP]
dc.contributor.authorNetto, Alcides Augusto Salzedas
dc.contributor.authorGonzalez, Adriano Miziara [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:59Z
dc.date.available2015-06-14T13:46:59Z
dc.date.issued2014-03-01
dc.description.abstractPURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.en
dc.description.affiliationSão Paulo Federal University
dc.description.affiliationUNIFESP Paulista School of Medicine Department of Surgery
dc.description.affiliationUNIFESP-EPM Department of Surgery
dc.description.affiliationUnifespSão Paulo Federal University
dc.description.affiliationUnifespUNIFESP, Paulista School of Medicine Department of Surgery
dc.description.affiliationUnifespUNIFESP, EPM Department of Surgery
dc.description.sourceSciELO
dc.format.extent171-177
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014000300005
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 171-177, 2014.
dc.identifier.doi10.1590/S0102-86502014000300005
dc.identifier.fileS0102-86502014000300171.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014000300171
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8286
dc.identifier.wosWOS:000333047100005
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiabetes Mellitus, Type 1en
dc.subjectIslets of Langerhans Transplantationen
dc.subjectOrgan Preservation Solutionsen
dc.subjectRatsen
dc.titleComparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in ratsen
dc.typeinfo:eu-repo/semantics/article
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