The expression of glycosaminoglycans and proteoglycans in the uterine cervix of albino rats after local hyaluronidase infusion

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dc.contributor.authorSouza, Guilherme Negrao [UNIFESP]
dc.contributor.authorCamano, Luiz [UNIFESP]
dc.contributor.authorAraujo Junior, Edward [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorMedeiros, Valquiria [UNIFESP]
dc.contributor.authorMaciel Martins, Joao Roberto [UNIFESP]
dc.contributor.authorSouza, Eduardo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:24Z
dc.date.available2016-01-24T14:37:24Z
dc.date.issued2014-06-01
dc.description.abstractObjective: To assess the local effect of hyaluronidase injection on the expression of glycosaminoglycans (GAGs) and proteoglycans (PGs) in the extracellular matrix of the uterine cervix from pregnant albino rats.Methods: Ten pregnant rats were divided into two groups on day 18 of pregnancy. the experimental group (Gexp) of rats received an intracervical infusion of 0.02 mL of hyaluronidase diluted to 1mL with distilled water, whereas the control group (Gc) received 1mL of distilled water. On day 20 of pregnancy, the pregnant rats were sacrificed and the uterine cervixes from all rats were then dissected. the qualitative expression of hyaluronic acid (HA) was assessed by immunohistochemistry and quantified by sandwich ELISA. To compare the quantitative GAG values between groups, a Student's t-test for independent samples was performed. PGs were also assessed by immunohistochemical analysis.Results: the electrophoretic profile of newly synthesized radioactively labeled GAGs degraded by specific enzymes showed that there were two predominant GAGs in both Gc and Gexp, i.e. heparan sulfate (HS) and a mixture of hondroitin sulfate (CS) and dermatan sulfate (DS). the concentrations of GAGs showed a significant reduction of CS/DS (p<0.004) and HS (p<0.005) relative to Gc. HA staining was less intense in the lamina propria and area surrounding the blood vessels in Gexp compared to Gc. the HA contents were also significantly reduced (p<0.012).Conclusions: Intracervical hyaluronidase infusion promoted a significant reduction in the concentration of sulfated GAGs as assessed by both qualitative (histochemical) and quantitative (fluorometric) measurements of HA.en
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Obstet, BR-05303000 São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Biochem, BR-05303000 São Paulo, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Obstet, BR-05303000 São Paulo, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Biochem, BR-05303000 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent879-886
dc.identifierhttp://dx.doi.org/10.3109/14767058.2013.845159
dc.identifier.citationJournal of Maternal-fetal & Neonatal Medicine. London: Informa Healthcare, v. 27, n. 9, p. 879-886, 2014.
dc.identifier.doi10.3109/14767058.2013.845159
dc.identifier.issn1476-7058
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37852
dc.identifier.wosWOS:000336567700004
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofJournal of Maternal-fetal & Neonatal Medicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectCervical maturationen
dc.subjectextracellular matrixen
dc.subjectglycosaminoglycansen
dc.subjecthyaluronidaseen
dc.titleThe expression of glycosaminoglycans and proteoglycans in the uterine cervix of albino rats after local hyaluronidase infusionen
dc.typeinfo:eu-repo/semantics/article
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