Interaction of oligonucleotide-based amphiphilic block copolymers with cell membrane models

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dc.contributor.authorCaseli, L. [UNIFESP]
dc.contributor.authorPascholati, C. P.
dc.contributor.authorTeixeira, F.
dc.contributor.authorNosov, S.
dc.contributor.authorVebert, C.
dc.contributor.authorMueeller, A. H. E.
dc.contributor.authorOliveira, O. N.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Basel
dc.contributor.institutionUniv Bayreuth
dc.date.accessioned2016-01-24T14:05:07Z
dc.date.available2016-01-24T14:05:07Z
dc.date.issued2010-07-01
dc.description.abstractOligonucleotides have unique molecular recognition properties, being involved in biological mechanisms such as cell-surface receptor recognition or gene silencing. for their use in human therapy for drug or gene delivery, the cell membrane remains a barrier, but this can be obviated by grafting a hydrophobic tail to the oligonucleotide. Here we demonstrate that two oligonucleotides, one consisting of 12 guanosine units (G(12)), and the other one consisting of five adenosine and seven guanosine (A(5)G(7)) units, when functionalized with poly(butadiene), namely PB-G(12) and PB-A(5)G(7), can be inserted into Langmuir monolayers of dipalmitoyl phosphatidyl choline (DPPC), which served as a cell membrane model. PB-G(12) and PB-A(5)G(7) were found to affect the DPPC monolayer even at high surface pressures. the effects from PB-G(12) were consistently stronger, particularly in reducing the elasticity of the DPPC monolayers, which may have important biological implications. Multilayers of DPPC and nucleotide-based copolymers could be adsorbed onto solid supports, in the form of Y-type LB films, in which the molecular-level interaction led to lower energies in the vibrational spectra of the nucleotide-based copolymers. This successful deposition of solid films opens the way for devices to be produced which exploit the molecular recognition properties of the nucleotides. (C) 2010 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Lab Mat Hibridos, Diadema, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Fis Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Basel, Dept Chem, Basel, Switzerland
dc.description.affiliationUniv Bayreuth, NW 2, D-95440 Bayreuth, Germany
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Lab Mat Hibridos, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipSwiss National Science Foundation
dc.description.sponsorshipIDSwiss National Science Foundation: 200020-121822
dc.format.extent56-61
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2010.03.006
dc.identifier.citationJournal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 347, n. 1, p. 56-61, 2010.
dc.identifier.doi10.1016/j.jcis.2010.03.006
dc.identifier.issn0021-9797
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32676
dc.identifier.wosWOS:000278157700008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Colloid and Interface Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectOligonucleotide-based amphiphilic block copolymeren
dc.subjectDPPCen
dc.subjectLangmuir monolayersen
dc.subjectLangmuir-Blodgett filmsen
dc.subjectBiosensoren
dc.titleInteraction of oligonucleotide-based amphiphilic block copolymers with cell membrane modelsen
dc.typeinfo:eu-repo/semantics/article
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