Analysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosis

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dc.contributor.authorKopelman, Alexander [UNIFESP]
dc.contributor.authorGirão, Manoel João Batista Castello [UNIFESP]
dc.contributor.authorBonetti, Tatiana Carvalho de Souza [UNIFESP]
dc.contributor.authorCarvalho, Cristina Valletta de [UNIFESP]
dc.contributor.authorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.authorSchor, Eduardo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2019-07-22T15:46:52Z
dc.date.available2019-07-22T15:46:52Z
dc.date.issued2016
dc.description.abstractEndometriosis affects approximately 12% of reproductive-age women and is currently diagnosed using invasive laparoscopic surgery. Differences in gene expression in the eutopic endometrium between women with and without endometriosis have been reported, and determining the reproducibility of these genetic differences in the endocervical epithelium would represent an important step toward developing novel diagnostic strategies. In this study, we analyzed gene expression in the endocervical epithelium in women with and without moderate or severe endometriosis. Using RT2 Profiler PCR Arrays, we analyzed gene expression in endocervical epithelial cells from women with deep endometriosis (n = 4) and healthy women (n =6). Nine genes were identified as being upregulated: 5 cell cycle genes (cyclin B1 [CCNB1], cyclin G1 [CCNG1], cullin 1 [CUL1], general transcription factor IIH, polypeptide 1 [GTF2H1], and proliferating cell nuclear antigen [PCNA]), 3 cytokine genes (C3, chemokine (C-C motif) ligand 21 [CCL21], and chemokine (C-X-C motif) ligand 14 [CXCL14]) and 1 gene related to dendritic cell pathways (ICAM2), showing that differential gene expression is present in the endocervical epithelium of women with deep endometriosis.en
dc.description.affiliationFed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Pelv Pain & Endometriosis Unit, Rua Napoleao Barros,608 Vila Clementino, BR-04024002 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Gynecol, Mol & Metabol Lab, Escola Paulista Med EPM UNIFESP, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Pelv Pain & Endometriosis Unit, Rua Napoleao Barros,608 Vila Clementino, BR-04024002 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Gynecol, Mol & Metabol Lab, Escola Paulista Med EPM UNIFESP, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent1269-1274
dc.identifierhttps://dx.doi.org/10.1177/1933719116638179
dc.identifier.citationReproductive Sciences. Thousand Oaks, v. 23, n. 9, p. 1269-1274, 2016.
dc.identifier.doi10.1177/1933719116638179
dc.identifier.issn1933-7191
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/51137
dc.identifier.wosWOS:000382544000018
dc.language.isoeng
dc.publisherSage Publications Inc
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEndometriosisen
dc.subjectNoninvasive diagnosisen
dc.subjectGene expressionen
dc.subjectEndocervixen
dc.titleAnalysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosisen
dc.typeinfo:eu-repo/semantics/article
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