Treatment with triterpenic fraction purified from Baccharis uncinella leaves inhibits Leishmania (Leishmania) amazonensis spreading and improves Th1 immune response in infected mice

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dc.contributor.authorYamamoto, Eduardo Seiji
dc.contributor.authorCampos, Bruno Luiz S.
dc.contributor.authorLaurenti, Marcia Dalastra
dc.contributor.authorLago, Joao H. G. [UNIFESP]
dc.contributor.authorGrecco, Simone dos Santos
dc.contributor.authorCorbett, Carlos E. P.
dc.contributor.authorPassero, Luiz Felipe D.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2016-01-24T14:35:02Z
dc.date.available2016-01-24T14:35:02Z
dc.date.issued2014-01-01
dc.description.abstractThe current medications used to treat leishmaniasis have many side effects for patients; in addition, some cases of the disease are refractory to treatment. Therefore, the search for new leishmanicidal compounds is indispensable. Recently, it was demonstrated that oleanolic- and ursolic-containing fraction from Baccharis uncinella leaves eliminated the promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and L. (Viannia) braziliensis without causing toxic effects for J774 macrophages. Thus, the aim of the present work was to characterize the therapeutic effect of the triterpenic fraction in L. (L.) amazonensis-infected BALB/c mice. Oleanolic- and ursolic acid-containing fraction was extracted from B. uncinella leaves using organic solvents and chromatographic procedures. L. (L.) amazonensis-infected BALB/c mice were treated intraperitoneally with triterpenic fraction during five consecutive days with 1.0 and 5.0 mg/kg of triterpenic fraction, or with 10.0 mg/kg of amphotericin B drug. Groups of mice treated with the triterpenic fraction, presented with decreased lesion size and low parasitism of the skin-both of which were associated with high amounts of interleukin-12 and interferon gamma. the curative effect of this fraction was similar to amphotericin B-treated mice; however, the final dose, required to eliminate amastigotes, was lesser than amphotericin B. Moreover, triterpenic fraction did not cause microscopic alterations in liver, spleen, heart, lung, and kidney of experimental groups. This work suggests that this fraction possesses compounds that are characterized by leishmanicidal and immunomodulatory activities. From this perspective, the triterpenic fraction can be explored as a new therapeutic agent for use against American Tegumentar Leishmaniasis.en
dc.description.affiliationUniv São Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infeciosas, BR-01246000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 São Paulo, Brazil
dc.description.affiliationUniv Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210180 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2012/03903-9
dc.description.sponsorshipID: HCFMUSP-LIM50
dc.format.extent333-339
dc.identifierhttp://dx.doi.org/10.1007/s00436-013-3659-x
dc.identifier.citationParasitology Research. New York: Springer, v. 113, n. 1, p. 333-339, 2014.
dc.identifier.doi10.1007/s00436-013-3659-x
dc.identifier.issn0932-0113
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37226
dc.identifier.wosWOS:000329106500040
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofParasitology Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.titleTreatment with triterpenic fraction purified from Baccharis uncinella leaves inhibits Leishmania (Leishmania) amazonensis spreading and improves Th1 immune response in infected miceen
dc.typeinfo:eu-repo/semantics/article
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