Interaction of polysaccharide-protein complex from Agaricus blazei with Langmuir and Langmuir-Blodgett films of phospholipids

Página do item simplificado
dc.contributor.authorSchmidt, Thais F.
dc.contributor.authorPavinatto, Felippe J.
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.contributor.authorGonzaga, Maria L. C.
dc.contributor.authorSoares, Sandra A.
dc.contributor.authorRicardo, Nagila M. P. S.
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Fed Ceara
dc.date.accessioned2016-01-24T13:52:15Z
dc.date.available2016-01-24T13:52:15Z
dc.date.issued2009-02-01
dc.description.abstractThe use of natural substances in health applications may be hampered by the difficulties in establishing the mechanisms of action, especially at molecular-level. the protein-polysaccharide complex extracted from the mushroom Agaricus blazei Murill, referred to as CAb, has been considered for treating various diseases with probable interaction with cell membranes. in this study, we investigate the interaction between CAb and a cell membrane model represented by a Langmuir monolayer of dimyristoyl phosphatidic acid (DMPA). CAb affects the structural properties of DMPA monolayers causing expansion and increasing compressibility. in addition, interaction with DMPA polar heads led to neutralization of the electrical double layer, yielding a zero surface potential at large areas per molecule. CAb remained at the interface even at high surface pressures, which allowed transfer of Langmuir-Blodgett (LB) films onto solid supports with the CAb-DMPA mixture. the mass transferred, according to quartz crystal microbalance (QCM) measurements, increased linearly with the number of deposited layers. With UV-vis absorption, fluorescence and FTIR spectroscopies, we confirmed that the LB films contain polysaccharides, proteins and DMPA. Therefore, the CAb biological action must be attributed not only to polysaccharides but also to proteins in the complex. (C) 2008 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Diadema, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Fis Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Quim, Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESR IMMP
dc.description.sponsorshipFINED
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipRede Biomat (Brazil)
dc.format.extent84-89
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2008.10.024
dc.identifier.citationJournal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 330, n. 1, p. 84-89, 2009.
dc.identifier.doi10.1016/j.jcis.2008.10.024
dc.identifier.issn0021-9797
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31296
dc.identifier.wosWOS:000262229700012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Colloid and Interface Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectLangmuir filmsen
dc.subjectPolysaccharide-protein complexesen
dc.subjectMushroom Agaricus blazei Murillen
dc.subjectLangmuir-Blodgett filmsen
dc.subjectCell membraneen
dc.subjectDMPAen
dc.subjectAir-water interfaceen
dc.subjectDepositionen
dc.subjectCompressibilityen
dc.subjectIn-plane elasticityen
dc.titleInteraction of polysaccharide-protein complex from Agaricus blazei with Langmuir and Langmuir-Blodgett films of phospholipidsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICAQF - Artigos