Effect of primer selection on estimates of GB virus C (GBV-C) prevalence and response to antiretroviral therapy for optimal testing for GBV-C viremia

dc.contributor.authorSouza, I. E. [UNIFESP]
dc.contributor.authorAllen, J. B.
dc.contributor.authorXiang, J.
dc.contributor.authorKlinzman, D.
dc.contributor.authorDiaz, R.
dc.contributor.authorZhang, S.
dc.contributor.authorChaloner, K.
dc.contributor.authorZdunek, D.
dc.contributor.authorHess, G.
dc.contributor.authorWilliams, C. F.
dc.contributor.authorBenning, L.
dc.contributor.authorStapleton, J. T.
dc.contributor.institutionUniv Iowa
dc.contributor.institutionIowa City Vet Adm Med Ctr
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionRoche Diagnost GmbH
dc.contributor.institutionJohns Hopkins Univ
dc.description.abstractGB virus C (GBV-C; also called hepatitis G virus) is a common cause of infection associated with prolonged survival among HIV-infected individuals. the prevalences of GBV-C viremia vary widely in different studies, and there has been poor agreement among different laboratories performing GBV-C RNA detection in quality control studies. To determine the optimal method of measuring GBV-C RNA in clinical samples, samples obtained from 939 HIV-infected subjects were studied using reverse transcription (RT)-PCR methods amplifying four separate regions of the GBV-C genome. Primers amplifying the E2 coding region were 100% specific; however, their sensitivity was only 76.6%. in contrast, primers amplifying three additional conserved regions of the GBV-C genome (the 5' nontranslated region and the nonstructural protein-coding regions 3 and 5A) were more sensitive but produced higher rates of false-positive results. Using low-specificity primer sets influenced the significance of association between GBV-C viremia and response to antiretroviral therapy. Using a quantitative GBV-C RNA method, the GBV-C RNA concentration did not correlate with baseline or set point HIV RNA levels; however, a correlation between negative, low, and high GBV-C RNA levels and increasing reduction in HIV RNA following antiretroviral therapy was observed. Subjects with both GBV-C E2 antibody and viremia had significantly lower GBV-C RNA levels than did viremic subjects without E2 antibody. These studies demonstrate that accurate detection of GBV-C RNA by nested RT-PCR requires the use of primers representing multiple genome regions. Analyses based on testing with single primers do not lead to reliable conclusions about the association between GBV-C infection and clinical outcomes.en
dc.description.affiliationUniv Iowa, Roy & Lucille Carver Coll Med, Dept Internal Med, Iowa City, IA 52242 USA
dc.description.affiliationIowa City Vet Adm Med Ctr, Iowa City, IA USA
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Div Infect Dis, São Paulo, Brazil
dc.description.affiliationUniv Iowa, Coll Publ Hlth, Dept Biostat, Iowa City, IA USA
dc.description.affiliationRoche Diagnost GmbH, Penzberg, Germany
dc.description.affiliationRoche Diagnost GmbH, Mannheim, Germany
dc.description.affiliationNIAID, Epidemiol Branch, Div AIDS, NIH,Dept Hlth & Human Serv, Bethesda, MD 20892 USA
dc.description.affiliationJohns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Div Infect Dis, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationJournal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 44, n. 9, p. 3105-3113, 2006.
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal of Clinical Microbiology
dc.rightsAcesso aberto
dc.titleEffect of primer selection on estimates of GB virus C (GBV-C) prevalence and response to antiretroviral therapy for optimal testing for GBV-C viremiaen
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