The chronic blockade of angiotensin I-converting enzyme eliminates the sex differences of serum cytokine levels of spontaneously hypertensive rats

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dc.contributor.authorDalpiaz, Polyana Lima Meireles
dc.contributor.authorLamas, Aline Zandonadi
dc.contributor.authorCaliman, Izabela Facco
dc.contributor.authorMedeiros, Ana Raquel Santos de
dc.contributor.authorAbreu, Glaucia Rodrigues de
dc.contributor.authorMoyses, Margareth Ribeiro
dc.contributor.authorAndrade, Tadeu Uggere
dc.contributor.authorAlves, Márcio Fernando [UNIFESP]
dc.contributor.authorCarmona, Adriana Karaoglanovic [UNIFESP]
dc.contributor.authorBissoli, Nazare Souza
dc.contributor.institutionUniv Fed Espirito Santo
dc.contributor.institutionInst Fed Espirito Santo
dc.contributor.institutionCtr Univ Vila Velha
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:10Z
dc.date.available2016-01-24T14:31:10Z
dc.date.issued2013-02-01
dc.description.abstractSex hormones modulate the action of both cytokines and the renin-angiotensin system. However, the effects of angiotensin I-converting enzyme (ACE) on the proinflammatory and anti-inflammatory cytokine levels in male and female spontaneously hypertensive rats (SHR) are unclear. We determined the relationship between ACE activity, cytokine levels and sex differences in SHR. Female (F) and male (M) SHR were divided into 4 experimental groups each (n = 7): sham + vehicle (SV), sham + enalapril (10 mg/kg body weight by gavage), castrated + vehicle, and castrated + enalapril. Treatment began 21 days after castration and continued for 30 days. Serum cytokine levels (ELISA) and ACE activity (fluorimetry) were measured. Male rats exhibited a higher serum ACE activity than female rats. Castration reduced serum ACE in males but did not affect it in females. Enalapril reduced serum ACE in all groups. IL-10 (FSV = 16.4 +/- 1.1 pg/mL; MSV = 12.8 +/- 1.2 pg/mL), TNF-alpha (FSV = 16.6 +/- 1.2 pg/mL; MSV = 12.8 +/- 1 pg/mL) and IL-6 (FSV = 10.3 +/- 0.2 pg/mL; MSV = 7.2 +/- 0.2 pg/mL) levels were higher in females than in males. Ovariectomy reduced all cytokine levels and orchiectomy reduced IL-6 but increased IL-10 concentrations in males. Castration eliminated the differences in all inflammatory cytokine levels (IL-6 and TNF-alpha) between males and females. Enalapril increased IL-10 in all groups and reduced IL-6 in SV rats. in conclusion, serum ACE inhibition by enalapril eliminated the sexual dimorphisms of cytokine levels in SV animals, which suggests that enalapril exerts systemic anti-inflammatory and anti-hypertensive effects.en
dc.description.affiliationUniv Fed Espirito Santo, Dept Ciencias Fisiol, BR-29042755 Vitoria, ES, Brazil
dc.description.affiliationInst Fed Espirito Santo, Vitoria, ES, Brazil
dc.description.affiliationCtr Univ Vila Velha, Dept Farm, Vila Velha, ES, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent171-177
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20122472
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 46, n. 2, p. 171-177, 2013.
dc.identifier.doi10.1590/1414-431X20122472
dc.identifier.fileS0100-879X2013000200171.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2013000200171
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35903
dc.identifier.wosWOS:000317144100010
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSHRen
dc.subjectEnalaprilen
dc.subjectSexual dimorphismen
dc.subjectAngiotensin I-converting enzymeen
dc.subjectCytokines IL-10en
dc.subjectIL-6 and TNF-alphaen
dc.titleThe chronic blockade of angiotensin I-converting enzyme eliminates the sex differences of serum cytokine levels of spontaneously hypertensive ratsen
dc.typeinfo:eu-repo/semantics/article
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