Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method

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dc.contributor.authorCarvalho, Rodrigo Franco de
dc.contributor.authorSakata, Sonia Tatsumi
dc.contributor.authorSilva, Dalton Giovanni Nogueira da
dc.contributor.authorMori, Enio
dc.contributor.authorBrandão, Paulo Eduardo
dc.contributor.authorRichtzenhain, Leonardo José
dc.contributor.authorPozzi, Claudia Rodrigues
dc.contributor.authorArcaro, Juliana Rodrigues Pozzi
dc.contributor.authorMiranda, Mariana Santos de
dc.contributor.authorMazzuchelli-de-Souza, Jacqueline
dc.contributor.authorMelo, Thatiana Correa de [UNIFESP]
dc.contributor.authorComenale, Gabriela
dc.contributor.authorAssaf, Suely Lucia Muro Rais
dc.contributor.authorBeçak, Willy
dc.contributor.authorStocco, Rita de Cassia
dc.contributor.institutionSecretaria Estado Saude São Paulo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCAPTA Bovinos Leiteiros
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed,Integracao Latino Americana UNILA
dc.date.accessioned2016-01-24T14:30:52Z
dc.date.available2016-01-24T14:30:52Z
dc.date.issued2013-01-01
dc.description.abstractBovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types of BPV are currently characterized and classified into three distinct genera, associated with different pathological outcomes. the described BPV types as well as other putative ones have been demonstrated by molecular biology methods, mainly by the employment of degenerated PCR primers. Specifically, divergences in the nucleotide sequence of the L1 gene are useful for the identification and classification of new papillomavirus types. On the present work, a method based on the PCR-RFLP technique and DNA sequencing was evaluated as a screening tool, allowing for the detection of two relatively rare types of BPV in lesions samples from a six-year-old Holstein dairy cow, chronically affected with cutaneous papillomatosis. These findings point to the dissemination of BPVs with unclear pathogenic potential, since two relatively rare, new described BPV types, which were first characterized in Japan, were also detected in Brazil.en
dc.description.affiliationSecretaria Estado Saude São Paulo, Inst Butantan, Genet Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Lab Biol Mol Aplicada & Sorol, BR-05508270 São Paulo, Brazil
dc.description.affiliationCAPTA Bovinos Leiteiros, Agencia Paulista Tecnol Agronegocios, Inst Zootecnia, Ctr Pesquisa Pecuaria Leite, BR-13460000 Nova Odessa, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Fed,Integracao Latino Americana UNILA, Dept Biol, BR-85867970 Foz de Iguacu, PR, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação do Desenvolvimento Administrativo (PAP-FUNDAP)
dc.description.sponsorshipIDCNPq: 402539/2011-7
dc.format.extent7
dc.identifierhttps://dx.doi.org/10.1155/2013/270898
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2013.
dc.identifier.doi10.1155/2013/270898
dc.identifier.fileWOS000321634400001.pdf
dc.identifier.issn2314-6133
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/35685
dc.identifier.wosWOS:000321634400001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBiomed Research International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleBovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Methoden
dc.typeinfo:eu-repo/semantics/article
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