Is Nitric Oxide a Mediator of the Effects of Low-Intensity Electrical Stimulation on Bone in Ovariectomized Rats?

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dc.contributor.authorLirani-Galvão, Ana Paula Rebucci [UNIFESP]
dc.contributor.authorLazaretti-Castro, Marise [UNIFESP]
dc.contributor.authorPortero-Muzy, N.
dc.contributor.authorBergamaschi, Cassia Toledo [UNIFESP]
dc.contributor.authorSilva, O. L.
dc.contributor.authorCarvalho, Aluizio Barbosa de [UNIFESP]
dc.contributor.authorDelmas, P. D.
dc.contributor.authorChavassieux, P.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Med RTH Laennec
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:05:06Z
dc.date.available2016-01-24T14:05:06Z
dc.date.issued2010-07-01
dc.description.abstractLow-intensity electrical stimulation (LIES) may counteract the effects of ovariectomy (OVX) on nitric oxide synthase (NOS) expression, osteocyte viability, bone structure, and microarchitecture in rats (Lirani-Galvo et al., Calcif Tissue Int 84:502-509, 2009). the aim of the present study was to investigate if these effects of LIES could be mediated by NO. We analyzed the effects of NO blockage (by l-NAME) in the response to LIES on osteocyte viability, bone structure, and microarchitecture in OVX rats. Sixty rats (200-220 g) were divided into six groups: sham, sham-l-NAME (6 mg/kg/day), OVX, OVX-l-NAME, OVX-LIES, and OVX-LIES-l-NAME. After 12 weeks, rats were killed and tibiae collected for histomorphometric analysis and immunohistochemical detection of endothelial NOS (eNOS), inducible NOS (iNOS), and osteocyte apoptosis (caspase-3 and TUNEL). in the presence of l-NAME, LIES did not counteract the OVX-induced effects on bone volume and trabecular number (as on OVX-LIES). l-NAME blocked the stimulatory effects of LIES on iNOS and eNOS expression of OVX rats. Both l-NAME and LIES decreased osteocyte apoptosis. Our results showed that in OVX rats l-NAME partially blocks the effects of LIES on bone structure, turnover, and expression of iNOS and eNOS, suggesting that NO may be a mediator of some positive effects of LIES on bone.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, BR-04039002 São Paulo, Brazil
dc.description.affiliationFac Med RTH Laennec, INSERM, U831, F-69372 Lyon 08, France
dc.description.affiliationUniv São Paulo, BR-13566590 Sao Carlos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, BR-04039002 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent52-59
dc.identifierhttps://dx.doi.org/10.1007/s00223-010-9357-0
dc.identifier.citationCalcified Tissue International. New York: Springer, v. 87, n. 1, p. 52-59, 2010.
dc.identifier.doi10.1007/s00223-010-9357-0
dc.identifier.issn0171-967X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32664
dc.identifier.wosWOS:000278929700006
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCalcified Tissue International
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectElectrical stimulationen
dc.subjectBone architectureen
dc.subjectOvariectomyen
dc.subjectNitric oxideen
dc.subjectL-NAMEen
dc.subjectOsteocyteen
dc.titleIs Nitric Oxide a Mediator of the Effects of Low-Intensity Electrical Stimulation on Bone in Ovariectomized Rats?en
dc.typeinfo:eu-repo/semantics/article
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