Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species

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dc.contributor.authorFernandes, Geisa Ferreira [UNIFESP]
dc.contributor.authorSantos, Priscila Oliveira dos [UNIFESP]
dc.contributor.authorRodrigues, Anderson Messias [UNIFESP]
dc.contributor.authorSasaki, Alexandre Augusto [UNIFESP]
dc.contributor.authorBurger, Eva
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Alfenas
dc.date.accessioned2016-01-24T14:31:29Z
dc.date.available2016-01-24T14:31:29Z
dc.date.issued2013-04-01
dc.description.abstractA comparative study about protein secretion, immunogenicity and virulence was performed in order to characterize and to compare eight Sporothrix schenckii sensu stricto isolates. for virulence characterization, a murine model, based on survival assay and CFU counting was used. S. brasiliensis and S. globosa, a highly virulent and a non-virulent isolates, respectively were used as external controls. Exoantigen profiles showed different secreted molecules; the 46- and 60-kDa molecules were commonly secreted by all three species. the S. schenckii s. str. isolates could be classified as non-virulent or presenting low, medium or high virulence, based on survival times after infection and recovery of viable fungi. the humoral response profiles of mice infected with S. schenckii s. str., S. globosa and S. brasiliensis were heterogeneous; five virulent isolates (S. schenckii s. str., n = 4 and S. brasiliensis, n = 1) had in common the recognition of the 60-kDa molecule by their respective antisera, suggesting that this antigen may be involved in virulence. Furthermore, the 110-kDa molecule was secreted and recognized by antisera from four virulent isolates (S. schenckii s. str., n = 3 and S. brasiliensis, n = 1), so there is a possibility that this molecule is also related to virulence. Our findings reveal different degrees of virulence in S. schenckii s. str. isolates and suggest the correlation of protein secretion and immunogenicity with virulence of S. schenckii complex. These findings provide new insights into the pathogenesis of S. schenckii s. str. and improve the knowledge about immunogenicity and protein profiles in S. schenckii complex.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil
dc.description.affiliationUniv Fed Alfenas, Dept Microbiol & Immunol, Inst Biomed Sci, Alfenas, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2009/54024-2
dc.format.extent241-249
dc.identifierhttp://dx.doi.org/10.4161/viru.23112
dc.identifier.citationVirulence. Austin: Landes Bioscience, v. 4, n. 3, p. 241-249, 2013.
dc.identifier.doi10.4161/viru.23112
dc.identifier.issn2150-5594
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36130
dc.identifier.wosWOS:000316948000006
dc.language.isoeng
dc.publisherLandes Bioscience
dc.relation.ispartofVirulence
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSporothrix schenckii complexen
dc.subjectvirulenceen
dc.subjectsporotrichosisen
dc.subjectantigensen
dc.subjectwestern blottingen
dc.subjectprotein secretionen
dc.subjectimmunogenicityen
dc.titleCharacterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis speciesen
dc.typeinfo:eu-repo/semantics/article
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