Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

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dc.contributor.authorMarques, Geraldo Magela Nogueira
dc.contributor.authorRasslan, Roberto
dc.contributor.authorBelon, Alessandro Rodrigo
dc.contributor.authorCarvalho, Juliana Gonçalves
dc.contributor.authorFelice Neto, Raphael
dc.contributor.authorRasslan, Samir
dc.contributor.authorUtiyama, Edivaldo Massazo
dc.contributor.authorMontero, Edna Frasson de Souza [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:47:22Z
dc.date.available2015-06-14T13:47:22Z
dc.date.issued2014-11-01
dc.description.abstractPURPOSE:To evaluate intestinal inflammatory and apoptotic processes after intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic saline.METHODS:It was allocated into four groups (n=6), 24 male Wistar rats (200 to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80 min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV); PTX group (Pentoxifylline, 30mg/kg-IV); HS+PTX group (Hypertonic Saline and Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion, ileal fragments were removed and stained on hematoxylin-eosin and histochemical studies for COX-2, Bcl-2 and cleaved caspase-3.RESULTS:The values of sO2 were higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40 minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098). Morphologic tissue injuries showed higher grades on IR group versus other groups: HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR).CONCLUSION:The combined use of pentoxifylline and hypertonic saline offers best results on inflammatory and apoptotic inhibitory aspects after intestinal ischemia/reperfusion.en
dc.description.affiliationSão Paulo University Medical School
dc.description.affiliationUSP Medical School
dc.description.affiliationFederal University of São Paulo Medical School
dc.description.affiliationUSP School of Medicine
dc.description.affiliationUSP School of Medicine Department of Surgery
dc.description.affiliationUSP Medical School Department of Surgery
dc.description.affiliationUnifespUNIFESP, Medical School
dc.description.sourceSciELO
dc.format.extent735-741
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001800007
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 11, p. 735-741, 2014.
dc.identifier.doi10.1590/S0102-86502014001800007
dc.identifier.fileS0102-86502014001100735.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001100735
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8651
dc.identifier.wosWOS:000345426500007
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectReperfusionen
dc.subjectIschemiaen
dc.subjectSaline Solution, Hypertonicen
dc.subjectPentoxifyllineen
dc.subjectApoptosisen
dc.subjectInflammationen
dc.subjectRatsen
dc.titlePentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in ratsen
dc.typeinfo:eu-repo/semantics/article
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