Identification of differentially expressed genes in pathways of cerebral neurotransmission of anovulatory mice

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dc.citation.issue3
dc.citation.volume16
dc.contributor.authorAzevedo, M. A., Jr. [UNIFESP]
dc.contributor.authorSilva, I. D. C. G. [UNIFESP]
dc.coverageRibeirao Preto
dc.date.accessioned2020-08-04T13:39:53Z
dc.date.available2020-08-04T13:39:53Z
dc.date.issued2017
dc.description.abstractPolycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with insulin resistance. The fact that these animals exhibit obesity, insulin resistance, and infertility demonstrates their skill as an experimental model for polycystic ovary. A recent study using long protocol for up to 40 weeks showed that anovulatory and obese mice transplanted with adipose tissue from animals with normal weight have multiple changes in their phenotype. These changes include reduction of body weight, prevention of obesity, insulin level normalization, and insulin tolerance tests, preventing the elevation of steroids and especially the reversal of fertility restoration with anovulation. Considering that there are close relationships between the ovulation process and the central nervous system, we propose to evaluate the gene expression levels of 84 different genes involved in neurotransmission and insulin pathways in addition to examining the neurolipidosis differential murine brain before and after reversal of anovulation. The present study showed changes in gene expression of molecular markers in brain tissue of animals for brain neurotransmission pathways as well as pathways for insulin. GABAergic genes, muscarinic, serotonin receptors, receptor tyrosine kinase, and genes of interleukin 6 showed overexpression profile. There was also a change in the lipid content in anovulatory brain, obesity, and insulin resistant mice (Ob-/Ob-) compared with controls. The re-introduction of leptin in these animals appears to reverse, at least in part, this profile.en
dc.description.affiliationUniv Fed Sao Paulo, Lab Ginecol Mol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Ginecol Mol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.4238/gmr16039622
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto, v. 16, n. 3, p. -, 2017.
dc.identifier.doi10.4238/gmr16039622
dc.identifier.fileWOS000417365000007.pdf
dc.identifier.issn1676-5680
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57161
dc.identifier.wosWOS:000417365000007
dc.language.isoeng
dc.publisherFunpec-Editora
dc.relation.ispartofGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDifferentially expressed genesen
dc.subjectNeurotransmitteren
dc.subjectAnovulationen
dc.titleIdentification of differentially expressed genes in pathways of cerebral neurotransmission of anovulatory miceen
dc.typeinfo:eu-repo/semantics/article
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