Dorsomedial hypothalamus CRF type 1 receptors selectively modulate inhibitory avoidance responses in the elevated T-maze

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dc.contributor.authorSilva, Mariana Santos Carvalho de Faria [UNIFESP]
dc.contributor.authorPereira, Bruno Aranha [UNIFESP]
dc.contributor.authorCéspedes, Isabel Cristina [UNIFESP]
dc.contributor.authorNascimento, Juliana Olivetti Guzman [UNIFESP]
dc.contributor.authorBittencourt, Jackson C.
dc.contributor.authorViana, Milena de Barros [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:37:48Z
dc.date.available2016-01-24T14:37:48Z
dc.date.issued2014-09-01
dc.description.abstractCorticotropin-releasing factor (CRF) plays a critical role in the mediation of physiological and behavioral responses to stressors. in the present study, we investigated the role played by the CRF system within the dorsomedial hypothalamus (DMH) in the modulation of anxiety- and panic-related responses. Male Wistar rats were administered into the DMH with CRF (125 and 250 ng/0.2 mu l, experiment 1) or with the CRFR1 antagonist antalarmin (25 ng/0.2 mu l, experiment 2) and 10 min later tested in the elevated T-maze (ETM) for inhibitory avoidance and escape measurements. in clinical terms, these responses have been respectively related to generalized anxiety and panic disorder. To further verify if the anxiogenic effects of CRF were mediated by CRFR1 activation, we also investigated the effects of the combined treatment with CRF (250 ng/0.2 mu l) and antalarmin (25 ng/0.2 mu l) (experiment 3). All animals were tested in an open field, immediately after the ETM, for locomotor activity assessment. Results showed that 250 ng/0.2 mu l of CRF facilitated ETM avoidance, an anxiogenic response. Antalarmin significantly decreased avoidance latencies, an anxiolytic effect, and was able to counteract the anxiogenic effects of CRF. None of the compounds administered altered escape responses or locomotor activity measurements. These results suggest that CRF in the DMH exerts anxiogenic effects by activating type 1 receptors, which might be of relevance to the physiopathology of generalized anxiety disorder. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psiquiatria & Psicol Med, BR-04038020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psiquiatria & Psicol Med, BR-04038020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2011/17471-0
dc.description.sponsorshipIDFAPESP: 2013/17389-8
dc.format.extent249-257
dc.identifierhttps://dx.doi.org/10.1016/j.bbr.2014.06.018
dc.identifier.citationBehavioural Brain Research. Amsterdam: Elsevier B.V., v. 271, p. 249-257, 2014.
dc.identifier.doi10.1016/j.bbr.2014.06.018
dc.identifier.issn0166-4328
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/38156
dc.identifier.wosWOS:000340323000034
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBehavioural Brain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCorticotropin-releasing factoren
dc.subjectCRFen
dc.subjectDorsomedial hypothalamusen
dc.subjectElevated T-mazeen
dc.subjectAnxietyen
dc.subjectPanicen
dc.titleDorsomedial hypothalamus CRF type 1 receptors selectively modulate inhibitory avoidance responses in the elevated T-mazeen
dc.typeinfo:eu-repo/semantics/article
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