Effects of Allogeneic Bone Marrow Derived Mesenchymal Stromal Cell Therapy on Voiding Function in a Rat Model of Parkinson Disease

Página do item simplificado
dc.contributor.authorCampeau, Lysanne
dc.contributor.authorSoler, Roberto [UNIFESP]
dc.contributor.authorSittadjody, Sivanandane
dc.contributor.authorPareta, Rajesh
dc.contributor.authorNomiya, Masanori
dc.contributor.authorZarifpour, Mona
dc.contributor.authorOpara, Emmanuel C.
dc.contributor.authorYoo, James J.
dc.contributor.authorAndersson, Karl-Erik
dc.contributor.institutionWake Forest Univ
dc.contributor.institutionNYU
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFukushima Med Univ
dc.date.accessioned2016-01-24T14:35:23Z
dc.date.available2016-01-24T14:35:23Z
dc.date.issued2014-03-01
dc.description.abstractPurpose: Cellular therapy induced transient urodynamic improvement in a rat model of Parkinson disease in which bladder dysfunction was noted after unilateral injection of 6-hydroxydopamine into the medial forebrain bundle. We sought to prolong the effect by injecting allogeneic rat bone marrow mesenchymal stromal cells before and after microencapsulation into the substantia nigra pars compacta.Materials and Methods: Female rats underwent unilateral stereotactic injection of 6-hydroxydopamine in the medial forebrain bundle. Injection was performed in the ipsilateral substantia nigra pars compacta using vehicle alone or vehicle with nonmicroencapsulated or microencapsulated rat bone marrow derived mesenchymal stromal cells. Rats were evaluated by cystometry 7, 14, 28 and 42 days after treatment. Brains were extracted for immunostaining.Results: At 42 days the nonmicroencapsulated group had lower threshold and intermicturition pressure, spontaneous activity and AUC than vehicle treated animals. Rats that received microencapsulated cells had lower threshold pressure at 28 days and lower spontaneous activity at 42 days than vehicle treated rats. Microencapsulated and nonmicroencapsulated rat bone marrow derived mesenchymal stromal cells were noted in the substantia nigra pars compacta up to 42 days after transplantation. At 42 days tyrosine hydroxylase positive neurons were more numerous in the substantia nigra pars compacta of the nonmicroencapsulated group, followed by the microencapsulated and vehicle treated groups.Conclusions: Urodynamic effects of the 6-hydroxydopamine lesion persisted up to 42 days after vehicle injection. Transplantation of nonmicroencapsulated rat bone marrow derived mesenchymal stromal cells improved urodynamic pressure by 42 days after treatment more markedly than microencapsulated cells. This was associated with more tyrosine hydroxylase positive neurons in the treated substantia nigra pars compacta of the nonmicroencapsulated group, suggesting that functional improvement requires a juxtacrine effect.en
dc.description.affiliationWake Forest Univ, Bowman Gray Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC USA
dc.description.affiliationNYU, Dept Urol, New York, NY USA
dc.description.affiliationUniversidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.description.affiliationFukushima Med Univ, Dept Urol, Sch Med, Fukushima, Japan
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Urol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipAmerican Urological Association Foundation
dc.description.sponsorshipAmerican Urological Association Southeastern Section Research Scholar Endowment Fund
dc.format.extent850-859
dc.identifierhttp://dx.doi.org/10.1016/j.juro.2013.08.026
dc.identifier.citationJournal of Urology. New York: Elsevier B.V., v. 191, n. 3, p. 850-859, 2014.
dc.identifier.doi10.1016/j.juro.2013.08.026
dc.identifier.issn0022-5347
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37493
dc.identifier.wosWOS:000331123400096
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Urology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjecturinary bladder, neurogenicen
dc.subjectParkinson diseaseen
dc.subjectmesenchymal stromal cellsen
dc.subjecturodynamicsen
dc.subjectsubstantia nigraen
dc.titleEffects of Allogeneic Bone Marrow Derived Mesenchymal Stromal Cell Therapy on Voiding Function in a Rat Model of Parkinson Diseaseen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos