Modeling epileptogenesis and temporal lobe epilepsy in a non-human primate

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dc.contributor.authorPerez-Mendes, P. [UNIFESP]
dc.contributor.authorBlanco, M. M. [UNIFESP]
dc.contributor.authorCalcagnotto, M. E. [UNIFESP]
dc.contributor.authorCinini, S. M. [UNIFESP]
dc.contributor.authorBachiega, J. [UNIFESP]
dc.contributor.authorPapoti, D. [UNIFESP]
dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.contributor.authorTannus, A. [UNIFESP]
dc.contributor.authorMello, L. E. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:09Z
dc.date.available2016-01-24T14:17:09Z
dc.date.issued2011-09-01
dc.description.abstractHere we describe a new non-human primate model of temporal lobe epilepsy (TLE) to better investigate the cause/effect relationships of human TLE. Status epilepticus (SE) was induced in adult marmosets by pilocarpine injection (250 mg/kg; i.p.). the animals were divided in 2 groups: acute (8 h post-SE) and chronic (3 and 5 months post-SE). To manage the severity of SE, animals received diazepam 5 min after the SE onset (acute group: 2.5 or 1.25 mg/kg; i.p.; chronic group/; 1.25 mg/kg; i.p). All animals were monitored by video and electrocorticography to assess SE and subsequent spontaneous recurrent seizures (SRS). To evaluate brain injury produced by SE or SRS we used argyrophil III, Nissl and neo-Timm staining techniques. Magnetic resonance image was also performed in the chronic group. We observed that pilocarpine was able to induce SE followed by SRS after a variable period of time. Prolonged SE episodes were associated with brain damage, mostly confined to the hippocampus and limbic structures. Similar to human TLE, anatomical disruption of dentate gyrus was observed after SRS. Our data suggest that pilocarpine marmoset model of epilepsy has great resemblance to human TLE, and could provide new tools to further evaluate the subtle changes associated with human epilepsy. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Physiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Inst Fis Sao Carlos IFSC, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Physiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Inst Fis Sao Carlos IFSC, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent45-57
dc.identifierhttp://dx.doi.org/10.1016/j.eplepsyres.2011.04.015
dc.identifier.citationEpilepsy Research. Amsterdam: Elsevier B.V., v. 96, n. 1-2, p. 45-57, 2011.
dc.identifier.doi10.1016/j.eplepsyres.2011.04.015
dc.identifier.issn0920-1211
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34006
dc.identifier.wosWOS:000295706700006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEpilepsy Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectPilocarpineen
dc.subjectTemporal lobe epilepsyen
dc.subjectMarmosetsen
dc.subjectStatus epilepticusen
dc.titleModeling epileptogenesis and temporal lobe epilepsy in a non-human primateen
dc.typeinfo:eu-repo/semantics/article
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