Short communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cells

dc.contributor.authorGuimaraes, Ana Paula [UNIFESP]
dc.contributor.authorSa-Filho, Dercy Jose [UNIFESP]
dc.contributor.authorSucupira, Maria Cecilia [UNIFESP]
dc.contributor.authorJanini, Luiz Mario [UNIFESP]
dc.contributor.authorDiaz, Ricardo Sobhie [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:51:32Z
dc.date.available2016-01-24T13:51:32Z
dc.date.issued2008-07-01
dc.description.abstractGenotypic resistance is currently assessed through direct sequencing, which cannot detect resistant strains below 20%. We compared the genotypic resistance profile of virions and proviruses using population-based analysis and single genome sequencing of the protease region of the pol gene in samples collected from five individuals in whom indinavir monotherapy resulted in treatment failure. Single genome sequencing showed that not all strains present the same resistance mutations, which can be dispersed across different HIV genomes. the resistance profile found in plasma was very similar to that found in peripheral blood mononuclear cells (PBMCs), confirming the utility of assessing proviral DNA as for a means of determining antiretroviral resistance.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Infect Dis, BR-04020041 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Infect Dis, BR-04020041 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent967-971
dc.identifierhttp://dx.doi.org/10.1089/aid.2008.0020
dc.identifier.citationAids Research and Human Retroviruses. New Rochelle: Mary Ann Liebert Inc, v. 24, n. 7, p. 967-971, 2008.
dc.identifier.doi10.1089/aid.2008.0020
dc.identifier.issn0889-2229
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30787
dc.identifier.wosWOS:000258302000011
dc.language.isoeng
dc.publisherMary Ann Liebert Inc
dc.relation.ispartofAids Research and Human Retroviruses
dc.rightsAcesso restrito
dc.titleShort communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cellsen
dc.typeArtigo
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