alpha-Tocopherol induces hematopoietic stem/progenitor cell expansion and ERK1/2-mediated differentiation

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dc.contributor.authorNogueira-Pedro, Amanda [UNIFESP]
dc.contributor.authorBarbosa, Christiano Marcello Vaz [UNIFESP]
dc.contributor.authorSegreto, Helena Regina Comodo [UNIFESP]
dc.contributor.authorLungato, Lisandro [UNIFESP]
dc.contributor.authorD'Almeida, Vania [UNIFESP]
dc.contributor.authorMoraes, Andrea Aparecida Fátima Souza [UNIFESP]
dc.contributor.authorMiranda, Antonio [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar Julian [UNIFESP]
dc.contributor.authorFerreira, Alice Teixeira [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:29Z
dc.date.available2016-01-24T14:17:29Z
dc.date.issued2011-12-01
dc.description.abstractTocopherols promote or inhibit growth in different cell types. in the hematopoietic system, the radioprotective property of tocopherols is thought to act through the expansion of primitive hematopoietic cells. However, the mechanisms activated by tocopherols and which HPs are affected remain poorly understood. To better address these questions, mice were treated with alpha-tocopherol, and its effects were investigated in the BM microenvironment. alpha-Tocopherol induced increased proliferation in HSC/HP cells, leading to BM hyperplasia. in addition, differentiation to the granulocytic/monocytic lineage was enhanced by alpha-tocopherol treatment. alpha-Tocopherol treatment resulted in decreased basal phosphorylation of ERK1/2, PKC, and STAT-5 in HSC/HP cells. in contrast, alpha-tocopherol enhanced ERK1/2 activation in response to IL-3 stimulation in HSC/HP cells without altering the expression of IL-3Rs. Moreover, alpha-tocopherol-induced differentiation and ERK1/2 activation were abolished in mice pretreated with a MEK inhibitor (PD98059); however, pretreatment with PD98059 did not reduce the alpha-tocopherol-mediated increase in HSC/HP cells but instead, further enhanced their proliferation. Therefore, alpha-tocopherol induces expansion of HSC/HP cells by a nonidentified intracellular pathway and granulocytic/monocytic differentiation through ERK1/2 activation. J. Leukoc. Biol. 90: 1111-1117; 2011.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Oncol Clin & Expt, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psicobiol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Oncol Clin & Expt, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psicobiol, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.format.extent1111-1117
dc.identifierhttps://dx.doi.org/10.1189/jlb.0611282
dc.identifier.citationJournal of Leukocyte Biology. Bethesda: Federation Amer Soc Exp Biol, v. 90, n. 6, p. 1111-1117, 2011.
dc.identifier.doi10.1189/jlb.0611282
dc.identifier.issn0741-5400
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34261
dc.identifier.wosWOS:000298154400009
dc.language.isoeng
dc.publisherFederation Amer Soc Exp Biol
dc.relation.ispartofJournal of Leukocyte Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectProliferationen
dc.subjectIL-3en
dc.subjectMonocyteen
dc.subjectGranulocyteen
dc.titlealpha-Tocopherol induces hematopoietic stem/progenitor cell expansion and ERK1/2-mediated differentiationen
dc.typeinfo:eu-repo/semantics/article
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