Murine macrophages cultured with IL-4 acquire a phenotype similar to that of epithelioid cells from granulomatous inflammation

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dc.contributor.authorCipriano, Ivone Martins [UNIFESP]
dc.contributor.authorMariano, Mario [UNIFESP]
dc.contributor.authorFreymuller, Edna [UNIFESP]
dc.contributor.authorCarneiro, Celia Regina Whitaker [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:58Z
dc.date.available2016-01-24T12:33:58Z
dc.date.issued2003-08-01
dc.description.abstractEpithelioid cells (ECs) found in granulomas are thought to derive from mononuclear phagocytes. Although GM-CSF and/or IL-4 are known to promote cell differentiation their role in the development of ECs has never been demonstrated. Here we showed that mouse macrophages treated exclusively with recombinant IL-4 (rIL-4) differentiate into epithelioid-like cells. Macrophages cultivated with rIL-4 presented a fried-egg shape, and ultrastructural studies revealed membrane interdigitations, cytoplasmic vesicles, prominent Golgi complex, and rough endoplasmic reticulum. Compared with controls, rIL-4 treated cells displayed increased expression of MHC class II molecules and of Migration Inhibitory Factor-Related Protein-14. Whereas mannose receptor-mediated phagocytosis was increased, Fcgamma-receptor mediated phagocytosis and the production of nitric oxide were decreased in treated cultures. All these features overlap those reported for ECs from granulomatous lesions. in conclusion, treatment of mouse peritoneal macrophages with rIL-4 drives their in vitro differentiation to an epithelioid phenotype and provides a tool to investigate the biology of ECs.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent201-211
dc.identifierhttps://dx.doi.org/10.1023/A:1025084413767
dc.identifier.citationInflammation. New York: Kluwer Academic/plenum Publ, v. 27, n. 4, p. 201-211, 2003.
dc.identifier.doi10.1023/A:1025084413767
dc.identifier.issn0360-3997
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27353
dc.identifier.wosWOS:000184619700003
dc.language.isoeng
dc.publisherKluwer Academic/plenum Publ
dc.relation.ispartofInflammation
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEpithelioid cellsen
dc.subjectGranulomaen
dc.subjectMacrophagesen
dc.subjectIL-4en
dc.subjectGM-CSFen
dc.titleMurine macrophages cultured with IL-4 acquire a phenotype similar to that of epithelioid cells from granulomatous inflammationen
dc.typeinfo:eu-repo/semantics/article
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