Activation of P2Y(1) receptor triggers two calcium signaling pathways in bone marrow erythroblasts
| dc.contributor.author | Paredes-Gamero, E. J. | |
| dc.contributor.author | Craveiro, R. B. | |
| dc.contributor.author | Pesquero, J. B. | |
| dc.contributor.author | Franca, J. P. | |
| dc.contributor.author | Oshiro, MEM | |
| dc.contributor.author | Ferreira, A. T. | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T12:41:02Z | |
| dc.date.available | 2016-01-24T12:41:02Z | |
| dc.date.issued | 2006-03-18 | |
| dc.description.abstract | In this study, we describe the presence of P2 receptor subtypes and Ca2+ signaling in erythroblasts. ATP and ADP produced a biphasic increase of intracellular Ca2+ concentration ([Ca2+],), with an initial transient phase followed by a sustained phase. Reverse transcription polymerase chain reaction (RT-PCR) showed the expression of P2Y(1), P2Y(2) and P2Y(12). the selective P2Y(1) receptor antagonist 2'-deoxy-N-6-methyl-adenosine- 3',5'diphosphate (MRS2179) and the Gi protein inhibitor pertussis toxin blocked Ca2+ increase. the initial transient [Ca2+](i) increase phase was sensitive to the 1,4,5-inositol trisphosphate (IP3) receptor blocker 2-aminoethoxy-diphenylborate (2-APB), while the sustained phase was sensitive to the protein kinase C (PKC) inhibitor 2-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3(1H-indol-3-yl)-maleimide (GF109203X) and calcium calmodulin kinase 11 (CaMKII) inhibitor 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62). in addition, the PKC activator phorbol-12,13-dibutyrate (PDBu) produced increase of [Ca2+](i). Flow cytometry analysis showed the expression of Ca2+-dependent PKC alpha, beta I, gamma and phospho-CaMKII.These results suggest that the activation of the P2Y(1) receptor triggers two different [Ca2+]i increase pathways, one IP3-dependent and the other kinase-dependent. (c) 2006 Elsevier B.V. All rights reserved. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.format.extent | 30-38 | |
| dc.identifier | http://dx.doi.org/10.1016/j.ejphar.2006.01.010 | |
| dc.identifier.citation | European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 534, n. 1-3, p. 30-38, 2006. | |
| dc.identifier.doi | 10.1016/j.ejphar.2006.01.010 | |
| dc.identifier.issn | 0014-2999 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/28793 | |
| dc.identifier.wos | WOS:000236409600004 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | European Journal of Pharmacology | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | bone marrow | en |
| dc.subject | erythroblast | en |
| dc.subject | P2Y(1) | en |
| dc.subject | receptor | en |
| dc.subject | intracellular calcium | en |
| dc.subject | CaMKII | en |
| dc.subject | PKC | en |
| dc.title | Activation of P2Y(1) receptor triggers two calcium signaling pathways in bone marrow erythroblasts | en |
| dc.type | info:eu-repo/semantics/article |