Cytogenetic instability of dental pulp stem cell lines

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dc.contributor.authorDuailibi, Monica Talarico [UNIFESP]
dc.contributor.authorKulikowski, Leslie Domenici
dc.contributor.authorDuailibi, Silvio Eduardo [UNIFESP]
dc.contributor.authorNunes Lipay, Monica Vannucci [UNIFESP]
dc.contributor.authorMelaragno, Maria Isabel
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.authorVacanti, Joseph Phillip
dc.contributor.authorYelick, Pamela Crotty
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionINCT Biofabris
dc.contributor.institutionMassachusetts Gen Hosp
dc.contributor.institutionHarvard Univ
dc.contributor.institutionDept Surg
dc.contributor.institutionTufts Univ
dc.date.accessioned2016-01-24T14:17:51Z
dc.date.available2016-01-24T14:17:51Z
dc.date.issued2012-02-01
dc.description.abstractHuman adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.en
dc.description.affiliationUniv São Paulo, Dept Pathol, Cytogenom Lab, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Plast Surg, Ctr Cellular & Mol Therapy, UNIFESP CTCMol, São Paulo, Brazil
dc.description.affiliationINCT Biofabris, Natl Inst Sci & Technol, Biofabricat Inst, São Paulo, Brazil
dc.description.affiliationUNIFESP Sao Jose Campos, Sci & Technol Inst Biomed Engn, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Endocrinol, São Paulo, Brazil
dc.description.affiliationMassachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 USA
dc.description.affiliationHarvard Univ, Sch Med, Boston, MA USA
dc.description.affiliationDept Surg, Boston, MA USA
dc.description.affiliationTufts Univ, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Plast Surg, Ctr Cellular & Mol Therapy, UNIFESP CTCMol, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP Sao Jose Campos, Sci & Technol Inst Biomed Engn, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Endocrinol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipINCT-Biofabrication Institute
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipRede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED
dc.description.sponsorshipNIH/NIDCR
dc.description.sponsorshipIDCNPq: 573661/2008-1
dc.description.sponsorshipIDFAPESP: 08/57860-3
dc.description.sponsorshipIDFAPESP: 07-58856-7
dc.description.sponsorshipIDFAPESP: 07-51227-4
dc.description.sponsorshipIDFAPESP: 07-59488-1
dc.description.sponsorshipIDRede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED: 208RT0340
dc.description.sponsorshipIDNIH/NIDCR: R01DE016132
dc.description.sponsorshipIDNIH/NIDCR: R03TW007665
dc.format.extent89-94
dc.identifierhttp://dx.doi.org/10.1007/s10735-011-9373-z
dc.identifier.citationJournal of Molecular Histology. Dordrecht: Springer, v. 43, n. 1, p. 89-94, 2012.
dc.identifier.doi10.1007/s10735-011-9373-z
dc.identifier.issn1567-2379
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34566
dc.identifier.wosWOS:000299376400011
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Molecular Histology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectChromosomal abnormalitiesen
dc.subjectCell transplantationen
dc.subjectTooth tissue engineeringen
dc.subjectHuman dental stem cellsen
dc.titleCytogenetic instability of dental pulp stem cell linesen
dc.typeinfo:eu-repo/semantics/article
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