Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

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dc.citation.issue12
dc.citation.volume11
dc.contributor.authorSantos, Uilson P.
dc.contributor.authorCampos, Jaqueline F.
dc.contributor.authorTorquato, Heron Fernandes Vieira [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar Julian [UNIFESP]
dc.contributor.authorCarollo, Carlos Alexandre
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorSouza, Kely de Picoli
dc.contributor.authordos Santos, Edson Lucas
dc.coverageSan Francisco
dc.date.accessioned2020-07-31T12:47:11Z
dc.date.available2020-07-31T12:47:11Z
dc.date.issued2016
dc.description.abstractHancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-dipheny1-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2'-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.en
dc.description.affiliationFed Univ Grande Dourados, Sch Environm & Biol Sci, Rodovia Dourados Ithaum, Dourados, MS, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Mogi Das Cruzes, Ctr Interdisciplinary Res Biochem, Av Dr Candido Xavier de Almeida Souza, Mogi Das Cruzes, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso do Sul, Ctr Biol & Hlth Sci, Lab Nat Prod & Mass Spectrometry, Campo Grande, MS, Brazil
dc.description.affiliationPolytech Inst Braganca, Agr Coll Braganca, Campus Santa Apolonia, Braganca, Portugal
dc.description.affiliationUniv Minho, Ctr Mol & Environm Biol, Campus Gualtar, Braga, Portugal
dc.description.affiliationUnifespDepartment of Biochemistry, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPrograma de Desenvolvimento Rural de Portugal (PRODER)
dc.description.sponsorshipIDPRODER: 24.073
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0167531
dc.identifier.citationPlos One. San Francisco, v. 11, n. 12, p. -, 2016.
dc.identifier.doi10.1371/journal.pone.0167531
dc.identifier.fileWOS000389482700185.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56646
dc.identifier.wosWOS:000389482700185
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsAcesso aberto
dc.titleAntioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomesen
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