Role of preoptic second messenger systems (cAMP and cGMP) in the febrile response

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dc.contributor.authorSteiner, A. A.
dc.contributor.authorAntunes-Rodrigues, J.
dc.contributor.authorBranco, LGS
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionDent Sch Ribeirao Preto
dc.date.accessioned2016-01-24T12:33:28Z
dc.date.available2016-01-24T12:33:28Z
dc.date.issued2002-07-19
dc.description.abstractThe present study aimed to test the hypothesis that a decrease in preoptic cAMP mediates fever. To this end, body core temperature (T-c) of unanesthetized, freely moving rats was monitored by biotelemetry before and after pharmacological modulation of the cAMP pathway, and cAMP levels in the anteroventral third ventricular region (AV3V), where the preoptic region (POA) is located, were determined. We observed that intra-POA administration of the cAMP agonist dibutyryl-cAMP (Db-cAMP, 40 mug) reduced T-c. PGE(2) (the proximal mediator of fever, 200 ng) raised T-c with a concomitant decrease in AV3V cAMP levels from 22.7+/-1.8 to 17.0+/-1.0 fmol/mug protein. Moreover, PGE(2)-induced fever was impaired by the phosphodiesterase inhibitor aminophylline. in order to verify the interaction between the cAMP- and cGMP-dependent pathways in the POA, we then co-injected Db-cAMP and 8-Br-cGMP into the POA. As a result, 8-Br-cGMP augmented the drop in 7 evoked by Db-cAMP. Lastly, we observed that intra-POA co-microinjection of the protein kinase A inhibitor (Rp-cAMPS, 1 mug) with the protein kinase G inhibitor (Rp-cGMPS, 1 mug), mimicking the effects of reduced production of cAMP and cGMP, respectively, produced a fever-like response. in summary, the present data support that a decrease in the levels of cAMP and cGMP in the POA is associated with the genesis of fever. (C) 2002 Elsevier Science BY. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med Ribeirao Preto, Dept Physiol, BR-14040904 Ribeirao Preto, SP, Brazil
dc.description.affiliationDent Sch Ribeirao Preto, Dept Morphol Estomatol & Physiol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med Ribeirao Preto, Dept Physiol, BR-14040904 Ribeirao Preto, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent135-145
dc.identifierhttp://dx.doi.org/10.1016/S0006-8993(02)02738-5
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 944, n. 1-2, p. 135-145, 2002.
dc.identifier.doi10.1016/S0006-8993(02)02738-5
dc.identifier.issn0006-8993
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26932
dc.identifier.wosWOS:000177224100014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectbody temperatureen
dc.subjectprostaglandinen
dc.subjectnitric oxideen
dc.subjectset pointen
dc.subjecthypothalamusen
dc.subjecttemperature-sensitive neuronen
dc.subjectcyclic AMPen
dc.subjectcyclic GMPen
dc.titleRole of preoptic second messenger systems (cAMP and cGMP) in the febrile responseen
dc.typeinfo:eu-repo/semantics/article
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