Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization

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dc.contributor.authorLo Turco, Edson Guimaraes [UNIFESP]
dc.contributor.authorMartins Ferreira Souza, Gustavo Henrique
dc.contributor.authorGarcia, Jerusa Simone
dc.contributor.authorFerreira, Christina Ramires
dc.contributor.authorEberlin, Marcos Nogueira
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionWaters Corp
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionAlfenas Fed Univ
dc.date.accessioned2016-01-24T13:59:48Z
dc.date.available2016-01-24T13:59:48Z
dc.date.issued2010-07-01
dc.description.abstractBACKGROUND: the aim of this study was to evaluate protein expression profile and quantify the proteins present in follicular fluid (FF) samples from women with endometriosis and pregnant women without endometriosis.METHODS: A prospective case control study was carried out including women with Stage III or IV endometriosis (Group I) and pregnant women without endometriosis (Group II), both at the maximum age of 35 years. Women were submitted to controlled ovarian stimulation for in vitro fertilization, and FF was collected after ultrasound-guided ovarian aspiration. FF from both ovaries was pooled, and patient samples were pooled according to Group I or II. Pooled protein samples were separated and analyzed by MudPIT (multidimensional protein identification technology followed by Expression(E) and label-free quantification with ProteinLynxGlobalServer 2.4v, Identity(E) and Expression(E) software).RESULTS: A total of 416 proteins or randomic sequence were identified, 62 proteins differentially expressed between Groups I and II. One ( 1.6%) was expressed at a higher level and 36 (58.1%) were uniquely expressed in Group 1, whereas 8 (12.9%) were expressed at a higher level and 17 (27.4%) were uniquely expressed in Group II. of all these, 15 (24.2%) are related to binding, I (1.6%) to immune response, 8 (12.9%) to cell division, 3 (4.8%) to cellular metabolism, 16 (25.8%) to general function and 19 (30.6%) do not yet present an identified function.CONCLUSIONS: Protein expression profiles of patients with and without endometriosis identified at least 64 proteins differentially expressed, which may be related to the physiopathology of endometriosis. These proteins may additionally be useful in determining potential biomarkers for diagnostics, as well as for therapeutic intervention in women with infertility due to endometriosis.en
dc.description.affiliationUniversidade Federal de São Paulo, Human Reprod Sect, Div Urol, Dept Surg, BR-04039060 São Paulo, Brazil
dc.description.affiliationWaters Corp, Mass Spectrometry Applicat Res & Dev Lab, BR-06455020 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationAlfenas Fed Univ, Dept Exact Sci, BR-37170000 Alfenas, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Human Reprod Sect, Div Urol, Dept Surg, BR-04039060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipDivision of Urology, Human Reproduction Section at the São Paulo Federal University
dc.description.sponsorshipThoMSon Mass Spectrometry Laboratory of the Institute of Chemistry at the University of Campinas
dc.description.sponsorshipIDCNPq: 475 108/2009-4
dc.description.sponsorshipIDFAPESP: 2008/10756-7
dc.format.extent1755-1766
dc.identifierhttp://dx.doi.org/10.1093/humrep/deq102
dc.identifier.citationHuman Reproduction. Oxford: Oxford Univ Press, v. 25, n. 7, p. 1755-1766, 2010.
dc.identifier.doi10.1093/humrep/deq102
dc.identifier.issn0268-1161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32641
dc.identifier.wosWOS:000280142800019
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofHuman Reproduction
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectbiomarkersen
dc.subjectendometriosisen
dc.subjectIVFen
dc.subjectfollicular fluiden
dc.subjectproteomicsen
dc.titleEffect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilizationen
dc.typeinfo:eu-repo/semantics/article
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