Cholesterol Mediates Chitosan Activity on Phospholipid Monolayers and Langmuir-Blodgett Films

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dc.contributor.authorPavinatto, Felippe J.
dc.contributor.authorPacholatti, Caue P.
dc.contributor.authorMontanha, Erica A.
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.contributor.authorSilva, Heurison S.
dc.contributor.authorMiranda, Paulo B.
dc.contributor.authorViitala, Tapani
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionKSV Instruments
dc.date.accessioned2016-01-24T13:58:43Z
dc.date.available2016-01-24T13:58:43Z
dc.date.issued2009-09-01
dc.description.abstractThe polysaccharide chitosan has been largely used in many biological applications as a fat and cholesterol reducer, bactericide agent, and wound healing material. While the efficacy for some of such uses is proven, little is known about the molecular-level interactions involved in these applications. in this study, we employ mixed Langmuir and Langmuir-Blodgett (LB) films of negatively charged dimyristoyl phosphatidic acid (DMPA) anti cholesterol as cell membrane models to investigate the role of cholesterol in the molecular-level action of chitosan. Chitosan does not remove cholesterol froth the monolayer. the interaction with chitosan tends to expand the DMPA monolayer due to its interpenetration within the film. On the other hand, cholesterol induces condensation of the DMPA monolayer. the competing effects cause the surface pressure isotherms of mixed DMPA-cholesterol films on a chitosan subphase to be unaffected by the cholesterol mole fraction, due to distinct degrees of chitosan penetration into the film in the presence of cholesterol. By combining polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and sum-frequency generation spectroscopy (SFG), we showed that chitosan induces order into negatively charged phospholipid layers, whereas the opposite occurs for cholesterol. in conclusion, chitosan has its penetration in the film modulated by cholesterol, and electrostatic interactions with negatively charged phospholipids, such as DMPA, are crucial for the action of chitosan.en
dc.description.affiliationUniv São Paulo, Inst Fis Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.affiliationKSV Instruments, Helsinki 00380, Finland
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPFSP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFinep (Brasil)
dc.format.extent10051-10061
dc.identifierhttp://dx.doi.org/10.1021/la901019p
dc.identifier.citationLangmuir. Washington: Amer Chemical Soc, v. 25, n. 17, p. 10051-10061, 2009.
dc.identifier.doi10.1021/la901019p
dc.identifier.issn0743-7463
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31793
dc.identifier.wosWOS:000269197500060
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofLangmuir
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleCholesterol Mediates Chitosan Activity on Phospholipid Monolayers and Langmuir-Blodgett Filmsen
dc.typeinfo:eu-repo/semantics/article
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