The plant-derived bauhinia bauhinioides kallikrein proteinase inhibitor (rbbki) attenuates elastase-induced emphysema in mice

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dc.contributor.authorMartins-Olivera, Bruno Tadeu
dc.contributor.authorAlmeida-Reis, Rafael
dc.contributor.authorTheodoro-Junior, Osmar Aparecido
dc.contributor.authorOliva, Leandro Vilela
dc.contributor.authordos Santos Nunes, Natalia Neto [UNIFESP]
dc.contributor.authorOlivo, Clarice Rosa
dc.contributor.authorde Brito, Marlon Vilela [UNIFESP]
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.contributor.authorLeick, Edna Aparecida
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorVilela Oliva, Maria Luiza [UNIFESP]
dc.contributor.authorRighetti, Renato Fraga
dc.contributor.authorLopes Calvo Tiberio, Iolanda de Fatima
dc.date.accessioned2019-01-21T10:29:52Z
dc.date.available2019-01-21T10:29:52Z
dc.date.issued2016
dc.description.abstractBackground. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2 alpha, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.en
dc.description.affiliationDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil
dc.description.affiliationDepartment of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil
dc.description.affiliationDepartment of Bioscience, Federal University of Sao Paulo, 11015-020 Santos, SP, Brazil
dc.description.affiliationUnifespDepartment of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Bioscience, Federal University of Sao Paulo, 11015-020 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq
dc.description.sponsorshipFAPESP [2013/17944-1]
dc.description.sponsorshipLIM-20 HC/FMUSP
dc.description.sponsorshipIDFAPESP: 2013/17944-1
dc.identifierhttp://dx.doi.org/10.1155/2016/5346574
dc.identifier.citationMediators Of Inflammation. New york, 2016.
dc.identifier.doi10.1155/2016/5346574
dc.identifier.fileWOS000381139500001.pdf
dc.identifier.issn0962-9351
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49448
dc.identifier.wosWOS:000381139500001
dc.language.isoeng
dc.publisherActa Cirurgica Brasileira
dc.relation.ispartofMediators Of Inflammation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectInduced Pulmonary-Emphysemaen
dc.subjectRho-Kinase Inhibitionen
dc.subjectChronic Inflammationen
dc.subjectPlasma Kallikreinen
dc.subjectAnimal-Modelsen
dc.subjectCopden
dc.subjectLungen
dc.subjectStressen
dc.subjectTissueen
dc.subjectProliferationen
dc.titleThe plant-derived bauhinia bauhinioides kallikrein proteinase inhibitor (rbbki) attenuates elastase-induced emphysema in miceen
dc.typeinfo:eu-repo/semantics/article
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