A novel protein phosphatase 2A (MA) is involved in the transformation of human protozoan parasite Trypanosoma cruzi

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dc.contributor.authorGonzalez, Jorge
dc.contributor.authorCornejo, Alberto
dc.contributor.authorSantos, Marcia Regina Machado [UNIFESP]
dc.contributor.authorCordero, Esteban Mauricio [UNIFESP]
dc.contributor.authorGutierrez, Bessy
dc.contributor.authorPorcile, Patricio
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.authorSagua, Hernan
dc.contributor.authorSilveira, José F. da [UNIFESP]
dc.contributor.authorAraya, Jorge E.
dc.contributor.institutionUniv Antofagasta
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:03Z
dc.date.available2016-01-24T12:34:03Z
dc.date.issued2003-09-14
dc.description.abstractHere we provide evidence for a critical role of PP2As (protein phosphatase 2As) in the transformation of Trypanosoma cruzi. in axenic medium at pH 5.0, trypomastigotes rapidly transform into amastigotes, a process blocked by okadaic acid, a potent PP2A inhibitor, at concentrations as low as 0.1 muM. 1-Norokadaone, an inactive okadaic acid analogue, did not affect the transformation. Electron microscopy studies indicated that okadaic acid-treated trypomastigotes had not undergone ultrastructural modifications, reinforcing the idea that PP2A inhibits transformation. Using a microcystin-Sepharose affinity column we purified the native T cruzi PP2A. the enzyme displayed activity against 12 P-labelled phosphorylase a that was inhibited in a dose-dependent manner by okadaic acid. the protein was also submitted to MS and, from the peptides obtained, degenerate primers were used to clone a novel T cruzi PP2A enzyme by PCR. the isolated gene encodes a protein of 303 amino acids, termed TcPP2A, which displayed a high degree of homology (86%) with the catalytic subunit of Trypanosoma brucei PP2A. Northern-blot analysis revealed the presence of a major 2.1-kb mRNA hybridizing in all T cruzi developmental stages. Southern-blot analysis suggested that the TcPP2A gene is present in low copy number in the T cruzi genome. These results are consistent with the mapping of PP2A genes in two chromosomal bands by pulsed-field gel electrophoresis and chromoblot hybridization. Our studies suggest that in T cruzi PP2A is important for the complete transformation of trypomastigotes into amastigotes during the life cycle of this protozoan parasite.en
dc.description.affiliationUniv Antofagasta, Dept Med Technol, Parasitol Unit, Antofagasta, Chile
dc.description.affiliationUniversidade Federal de São Paulo, EPM, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent647-656
dc.identifierhttp://dx.doi.org/10.1042/BJ20030215
dc.identifier.citationBiochemical Journal. London: Portland Press, v. 374, p. 647-656, 2003.
dc.identifier.doi10.1042/BJ20030215
dc.identifier.issn0264-6021
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27414
dc.identifier.wosWOS:000185546800007
dc.language.isoeng
dc.publisherPortland Press
dc.relation.ispartofBiochemical Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectenzyme purificationen
dc.subjectexpressionen
dc.subjectgene cloningen
dc.subjectgenomic organizationen
dc.subjectphosphatase-specific inhibitoren
dc.subjecttrypanosome transformationen
dc.titleA novel protein phosphatase 2A (MA) is involved in the transformation of human protozoan parasite Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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