Species diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazil

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dc.citation.issue5
dc.citation.volume60
dc.contributor.authorSilva, Wendy C. [UNIFESP]
dc.contributor.authorGoncalves, Sarah S.
dc.contributor.authorSantos, Daniel W. C. L. [UNIFESP]
dc.contributor.authorPadovan, Ana Carolina B. [UNIFESP]
dc.contributor.authorBizerra, Fernando C. [UNIFESP]
dc.contributor.authorMelo, Analy S. A. [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-17T14:02:13Z
dc.date.available2020-07-17T14:02:13Z
dc.date.issued2017
dc.description.abstractThe Exophiala genus is responsible for many superficial and invasive infections resulting from black fungi. Identification of Exophiala at the species level is based on morphological observations complemented by molecular tests. The aim of this study was to identify 23 clinical isolates of Exophiala spp. and evaluate the antifungal susceptibility to seven different agents. Molecular identification was based on an analysis of ITS region of rDNA using genomic databases. The micromorphology was evaluated by microculture and scanning electron microscopy. The susceptibility tests were performed using the antifungal agents 5-fluorocytosine (5-FC), amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), caspofungin (CFG) and terbinafine (TRB). The ITS analysis identified 100% of the following isolates as: E.dermatitidis (8), E.xenobiotica (6), E.bergeri (4), E.oligosperma (3), E.spinifera (1) and E.mesophila (1). The antifungal susceptibility tests showed that the triazoles compounds were in vitro the most active agents against Exophiala. ITS sequencing enabled the accurate identification of the 23 tested isolates. The triazoles, particularly itraconazole and posaconazole, exhibited MIC values lower than AMB, CAS and 5-FC. Although the guidelines do not indicate AMB for treatment against Exophiala spp., this study showed activity for all of the tested species, except E.mesophila.en
dc.description.affiliationUniv Fed Sao Paulo, Lab Especial Micol, Disciplina Infectol, Sao Paulo, Brazil
dc.description.affiliationFundacao Oswaldo Ramos, Hosp Rim, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Espirito Santo, CIMM, Dept Patol, Vitoria, Brazil
dc.description.affiliationUniv Fed Alfenas, Inst Ciencias Biomed, Dept Microbiol & Imunol, Alfenas, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Especial Micol, Disciplina Infectol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal em Nivel Superior
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDCAPES: PNPD 23038.007393/2011-11
dc.format.extent328-337
dc.identifierhttp://dx.doi.org/10.1111/myc.12597
dc.identifier.citationMycoses. Hoboken, v. 60, n. 5, p. 328-337, 2017.
dc.identifier.doi10.1111/myc.12597
dc.identifier.issn0933-7407
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54693
dc.identifier.wosWOS:000399002800005
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofMycoses
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectantifungal susceptibilityen
dc.subjectExophiala sppen
dc.subjectspecies identificationen
dc.titleSpecies diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazilen
dc.typeinfo:eu-repo/semantics/article
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